ScholarWorks Community:
https://scholarworks.sookmyung.ac.kr/handle/2021.sw.sookmyung/194
2024-03-27T19:09:14Z
2024-03-27T19:09:14Z
A lysosomal NO-selective fluorescent probe enables bioorthogonal exploration of NO involved in autophagy and ferroptosis
Park, Sun Young
Jo, Eun-Young
Kang, Chulhun
Lee, Min Hee
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/159749
2024-03-22T04:30:25Z
2024-03-01T00:00:00Z
Title: A lysosomal NO-selective fluorescent probe enables bioorthogonal exploration of NO involved in autophagy and ferroptosis
Authors: Park, Sun Young; Jo, Eun-Young; Kang, Chulhun; Lee, Min Hee
Abstract: Compartmentalized nitric oxide (NO) within specific organelles in cells has been associated with the selective expression of NO syntheses (NOSs) in these organelles. Moreover, it has been reported that lysosomal NOS expression contributes to autophagy and ferroptosis. Unfortunately, a method for detecting NO within lysosomes in live cells unavailable yet. In this study, we developed a lysosomal NO-selective chemosensor (Lyso-NO), composed of a 4-(4-nitrophenyl)thiosemicarbazide-linked naphthalimide as an NO-mediated fluorogenic reporter and a morpholine moiety as a lysosome-targeting unit. Upon selective reaction with NO in solution and within live cells, Lyso-NO showed a strong fluorescence intensity at 550 nm, and its predominant lysosomal location was revealed by confocal microscopy. Further, lysosomal NO increase was demonstrated to be deeply involved in autolysosome formation during autophagy, and in ferroptosis. Lyso-NO was considered to act as a lysosomal NO-selective sensor, allowing for the bioorthogonal analysis of lysosome-related biological processes. © 2023 Elsevier B.V.
2024-03-01T00:00:00Z
Synergistic Interaction between Ruthenium Catalysts and Grafted Niobium on SBA-15 for 2,5-Furandicarboxylic Acid Production Using 5-Hydroxymethylfurfural
Perumal, Santhana Krishnan
Lee, Sangyeob
Yu, Hyejin
Heo, Jaeseong
Kang, Myung Jong
Kim, Yeonjoon
Park, Myeongkee
Lee, Hangil
Kim, Hyun Sung
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/159762
2024-03-22T04:30:31Z
2024-02-01T00:00:00Z
Title: Synergistic Interaction between Ruthenium Catalysts and Grafted Niobium on SBA-15 for 2,5-Furandicarboxylic Acid Production Using 5-Hydroxymethylfurfural
Authors: Perumal, Santhana Krishnan; Lee, Sangyeob; Yu, Hyejin; Heo, Jaeseong; Kang, Myung Jong; Kim, Yeonjoon; Park, Myeongkee; Lee, Hangil; Kim, Hyun Sung
Abstract: This study entailed the synthesis of Ru nanocatalyst decorated on Nb-grafted SBA-15. A Nb-grafted SBA-15 support with varying Nb contents was utilized as a support for the Ru nanoparticles. The effect of Nb grafting on the immobilized Ru nanoparticle catalyst was systematically investigated, and its catalytic performance in the synthesis of furandicarboxylic acid using 5-hydroxymethylfurfural under base-free reaction conditions was evaluated. The results indicate the increased productivity of the Ru@Nb-grafted SBA-15 catalyst with a yield exceeding 95%, representing a significant advancement in catalysis. This study also affords insights into the complex relationship between the catalytic activity and selectivity and its unique surface attributes. Moreover, acidic sites were created, and the electron density within the active sites was modulated by monomeric Nb oxide species on the SBA-15. Additionally, the role of high-electron-density Ru atoms in facilitating the efficient adsorption and activation of the reactant, resulting in enhanced catalytic efficacy, was highlighted.
2024-02-01T00:00:00Z
Photo-oxidative Crack Propagation in Transition Metal Dichalcogenides
Ben-Smith, Andrew
Choi, Soo Ho
Boandoh, Stephen
Lee, Byung Hoon
Vu, Duc Anh
Nguyen, Huong Thi Thanh
Adofo, Laud Anim
Jin, Jeong Won
Kim, Soo Min
Lee, Young Hee
Kim, Ki Kang
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/159761
2024-03-22T04:30:29Z
2024-01-01T00:00:00Z
Title: Photo-oxidative Crack Propagation in Transition Metal Dichalcogenides
Authors: Ben-Smith, Andrew; Choi, Soo Ho; Boandoh, Stephen; Lee, Byung Hoon; Vu, Duc Anh; Nguyen, Huong Thi Thanh; Adofo, Laud Anim; Jin, Jeong Won; Kim, Soo Min; Lee, Young Hee; Kim, Ki Kang
Abstract: Monolayered transition-metal dichalcogenides (TMDs) are easily exposed to air, and their crystal quality can often be degraded via oxidation, leading to poor electronic and optical device performance. The degradation becomes more severe in the presence of defects, grain boundaries, and residues. Here, we report crack propagation in pristine TMD monolayers grown by chemical vapor deposition under ambient conditions and light illumination. Under a high relative humidity (RH) of similar to 60% and white light illumination, the cracks appear randomly. Photo-oxidative cracks gradually propagated along the grain boundaries of the TMD monolayers. In contrast, under low RH conditions of similar to 2%, cracks were scarcely observed. Crack propagation is predominantly attributed to the accumulation of water underneath the TMD monolayers, which is preferentially absorbed by hygroscopic alkali metal-based precursor residues. Crack propagation is further accelerated by the cyclic process of photo-oxidation in a basic medium, leading to localized tensile strain. We also found that such crack propagation is prevented after the removal of alkali metals via the transfer of the sample to other substrates.
2024-01-01T00:00:00Z
Low-Loaded Polyethylene Glycol (PEG) Resin for High-Purity Peptide Synthesis and Cell Binding Assays
Kim, Seongsoo
Park, Jeeyeon
Kim, Hye-Won
Kim, Jinsik
Bae, Joonwon
Lee, Min Hee
Shin, Dong-Sik
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/159744
2024-03-21T01:30:20Z
2023-12-01T00:00:00Z
Title: Low-Loaded Polyethylene Glycol (PEG) Resin for High-Purity Peptide Synthesis and Cell Binding Assays
Authors: Kim, Seongsoo; Park, Jeeyeon; Kim, Hye-Won; Kim, Jinsik; Bae, Joonwon; Lee, Min Hee; Shin, Dong-Sik
Abstract: In solid-phase peptide synthesis (SPPS), a high concentration of functional groups on the solid support and an adequate swelling volume enables its use for high-throughput screening of receptor agonists and therapeutic peptides. However, a high resin loading often leads to insufficient purity of the synthesized peptides and false positive binding with the target due to improper interactions between neighboring peptides. Therefore, this study focused on low-loaded polyethylene glycol (PEG) resins to achieve high-specificity screening using a core-shell-type PEG hydrogel resin. The peptide purity and target specificity were determined by assessing (1) the resin swelling properties in various solvents, (2) purity of a complicated Jung-Redemann (JR) decapeptide, and (3) the cell-adhesive behavior of GRGDS-pentapeptide-bound resins. The results were compared with those obtained using polyacrylamide resin (PAM) and conventionally used TentaGel S NH2 resin (TG((R))). The highest JR decapeptide purity was achieved using the PEG-based resin with a higher degree of cross-linking (PEGHN). Furthermore, the resin was preferably qualified as an extracellular microenvironment to accommodate true specific binding with fibroblast cells. Thus, SPPS and cell binding assays using the developed PEG-based resin provide a novel stringent strategy with potential application for true positive screening in biological assays.
2023-12-01T00:00:00Z