목단피로부터 식품부패 미생물에 대한 항균성 물질의 분리 및 동정Isolation and Identification of Antimicrobial Compound from Mokdan Bark (Paeonia suffruticosa ANDR)
- Other Titles
- Isolation and Identification of Antimicrobial Compound from Mokdan Bark (Paeonia suffruticosa ANDR)
- Authors
- 황재선; 한영실
- Issue Date
- Oct-2003
- Publisher
- 한국식품영양과학회
- Keywords
- antimicrobial activity; Mokdanbark (Paeonia suffruticosa ANDR); ethylacetate fraction; isobutyl isopentanoate; antimicrobial activity; Mokdanbark (Paeonia suffruticosa ANDR); ethylacetate fraction; isobutyl isopentanoate
- Citation
- 한국식품영양과학회지, v.32, no.7, pp 1059 - 1065
- Pages
- 7
- Journal Title
- 한국식품영양과학회지
- Volume
- 32
- Number
- 7
- Start Page
- 1059
- End Page
- 1065
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/10269
- ISSN
- 1226-3311
2288-5978
- Abstract
- Antimicrobial activity of Mokdan bark (Paeonia suffruticosa ANDR) was investigated. Methanol extract of dried Mokdan was fractionated to hexane, chloroform, ethylacetate, butanol and aqueous fraction. Ethylacetate fraction among these fractions showed the highest inhibitory effect on the microorganisms such as L. monocytogenes, and E. coli at 500 μg/disc. Ethylacetate fraction was further fractionated into 3 fractions by silica gel column and thin layer chromatography (TLC). The results showed that ethylacetate fractions No. 1 and 2 had the highest antimicrobial activity. They were mixed again, reseparated, and 3 fractions were obtained. Among them, No. 1 had the highest inhibitory effect on the microorganisms, No. 1 fraction was identified as isobutyl isopentanoate by HPLC, and GC-MS.
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