Simultaneous determination of 7-O-succinyl macrolactin A and its active major metabolite, macrolactin A in dog plasma using high-performance liquid chromatography with UV detection
- Authors
- Kim, Eunyoung; Shin, Beomsoo; Kwon, Kwang-il; Bang, Joon Seok; Kang, Wonku
- Issue Date
- Oct-2014
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- Dog plasma; High-performance liquid chromatography; Macrolactin A; 7-O-Succinyl macrolactin A; UV detection
- Citation
- JOURNAL OF SEPARATION SCIENCE, v.37, no.20, pp 2833 - 2836
- Pages
- 4
- Journal Title
- JOURNAL OF SEPARATION SCIENCE
- Volume
- 37
- Number
- 20
- Start Page
- 2833
- End Page
- 2836
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/10777
- DOI
- 10.1002/jssc.201400438
- ISSN
- 1615-9306
1615-9314
- Abstract
- We developed a method for the simultaneous quantification of 7-O-succinyl macrolactin A and its active metabolite, macrolactin A, in dog plasma. After protein precipitation with acetonitrile including flufenamic acid as an internal standard, 7-O-succinyl macrolactin A, macrolactin A, and flufenamic acid were chromatographed on a reverse-phase C-18 analytical column. The mobile phase, consisting of 20 mM acetate buffer and acetonitrile, was eluted using a gradient program at 1 mL/min, and the UV absorbance was measured at 230 nm. The retention times of 7-O-succinylmacrolactin A, flufenamic acid, and macrolactin A were 3.4, 4.8, and 6.9 min, respectively. The coefficient of variation in the assay precision for both substances was less than 6%, and the accuracy ranged from 96 to 105%. This method was used to measure the concentrations of 7-O-succinylmacrolactin A and macrolactin A in dog plasma following an intravenous administration of a single dose (25 mg/kg) of 7-O-succinyl macrolactin A salt.
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