Detailed Information

Cited 0 time in webofscience Cited 2 time in scopus
Metadata Downloads

Interleukin-32 alpha modulates promyelocytic leukemia zinc finger gene activity by inhibiting protein kinase C epsilon-dependent sumoylation

Authors
Park, Yun SunKang, Jeong-WooLee, Dong HunKim, Man SubBak, YesolYang, YoungLee, Hee-GuHong, JintaeYoon, Do-Young
Issue Date
Oct-2014
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Keywords
Interleukin 32 alpha; Promyelocytic leukemia zinc finger protein; Small ubiquitin-like modifier-2
Citation
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, v.55, pp 136 - 143
Pages
8
Journal Title
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
Volume
55
Start Page
136
End Page
143
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/10783
DOI
10.1016/j.biocel.2014.08.018
ISSN
1357-2725
1878-5875
Abstract
Interleukin-32 (IL-32) is a proinflammatory cytokine. However, there is growing evidence that IL-32 also plays a mediatory role intracellularly. In this study, we present evidence that IL-32 alpha modifies and inhibits promyelocytic leukemia zinc finger (PLZF), a sequence-specific transcriptional regulator that regulates the expression of a subset of interferon (IFN)-stimulated genes (ISGs). We screened IL-32 alpha-interacting proteins in a human spleen cDNA library using the yeast two-hybrid assay, and investigated the functional relevance of the interaction between IL-32 alpha and PLZF. We demonstrated that IL-32 alpha interacts with protein kinase C (PKC)delta and PKC epsilon in a phorbol 12-myristate 13-acetate (PMA) dependent way, and that PKC epsilon regulates the interaction of IL-32 alpha with PLZF. We verified the involvement of PKC epsilon in the interaction between these proteins by using various PKC inhibitors. PLZF is known to be modified by small ubiquitin-like modifier (SUMO)-1, but it is unclear whether SUMO-2 conjugation of PLZF occurs. We showed that IL-32 alpha inhibited SUMO-2-conjugation of PLZF. Further, we demonstrated that sumoylated PLZF decreased when IL-32 alpha was co-expressed. PKC epsilon affected the sumoylation of PLZF only in the presence of IL-32 alpha because PKC inhibitor treatment did not reduce PLZF sumoylation in the absence of IL-32 alpha. We finally investigated whether IL-32 alpha-mediated inhibition of PLZF sumoylation affected the transcriptional activity of PLZF, and demonstrated that the inhibition of sumoylation of PLZF by IL-32 alpha down-regulated ISGs induced by PLZF. Together, our data suggest that IL-32 alpha associates with PLZF and PKC epsilon, and then inhibits PLZF sumoylation, resulting in suppression of the transcriptional activity of PLZF. (C) 2014 Elsevier Ltd. All rights reserved.
Files in This Item
Go to Link
Appears in
Collections
이과대학 > 생명시스템학부 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Yang, Young photo

Yang, Young
이과대학 (생명시스템학부)
Read more

Altmetrics

Total Views & Downloads

BROWSE