Corticotropin-releasing factor induces immune escape of cervical cancer cells by downregulation of NKG2D
- Authors
- Song, Hyunkeun; Park, Hyunjin; Park, Gabin; Kim, Yeong Seok; Lee, Hyun-Kyung; Jin, Dong-Hoon; Kang, Hyung-Sik; Cho, Dae-Ho; Hur, Daeyoung
- Issue Date
- Jul-2014
- Publisher
- SPANDIDOS PUBL LTD
- Keywords
- corticotropin-releasing factor; NKG2D ligand; immune escape; natural killer cells
- Citation
- ONCOLOGY REPORTS, v.32, no.1, pp 425 - 430
- Pages
- 6
- Journal Title
- ONCOLOGY REPORTS
- Volume
- 32
- Number
- 1
- Start Page
- 425
- End Page
- 430
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/10857
- DOI
- 10.3892/or.2014.3191
- ISSN
- 1021-335X
1791-2431
- Abstract
- Corticotropin-releasing factor (CRF), a coordinator of the body's responses to stress, is found in various cancer tissues and cell lines. However, the exact abilities of CRF to manipulate natural killer (NK) cells during immune response have not been studied. NKG2D is an activating receptor that is expressed on most NK and CD8(+) T cells. MHC class I-related chain A (MICA) and UL16-binding protein (ULBP) 1,2 and 3 are well-known ligands for NKG2D. In the present study, we reported our findings regarding the role of CRF in cervical cancer cell survival. Human cervical cancer cell line, HeLa cells, had significantly higher intracellular expression of UL16-binding protein 2 (ULBP2) following CRF treatment but had only slightly increased surface expression of ULBP2. Notably, MMPi (pan-metalloproteases inhibitor) blocked the release of ULBP2 molecules from the surface of HeLa cells. Furthermore, incubating NK cells with culture supernatants from CRF-treated HeLa cells, which contained soluble NKG2D ligand, reduced NK cell activity by decreasing surface expression of NKG2D. Collectively, downregulation of NKG2D by CRF-induced soluble NKG2D ligand provides a potential mechanism by which cervical cancer cells escape NKG2D-mediated attack under stress conditions.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 대학 > 기초교양대학 > 기초교양학부 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.