Rapid Detection of Listeria monocytogenes by Real-Time PCR in Processed Meat and Dairy Products
- Authors
- Heo, Eun Jeong; Song, Bo Ra; Park, Hyun Jung; Kim, Young Jo; Moon, Jin San; Wee, Sung Hwan; Kim, Jin-Seok; Yoon, Yohan
- Issue Date
- Mar-2014
- Publisher
- INT ASSOC FOOD PROTECTION
- Citation
- JOURNAL OF FOOD PROTECTION, v.77, no.3, pp 453 - 458
- Pages
- 6
- Journal Title
- JOURNAL OF FOOD PROTECTION
- Volume
- 77
- Number
- 3
- Start Page
- 453
- End Page
- 458
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/10967
- DOI
- 10.4315/0362-028X.JFP-13-318
- ISSN
- 0362-028X
1944-9097
- Abstract
- The objectives of this study were to evaluate the detection of Listeria monocyto genes in different ready-to-eat foods using real-time PCR (RT-PCR). Various concentrations (10(0) to 10(5) CFU/ml) of L. monocytogenes ATCC 19115 were inoculated into ham, sausage, ground meat, processed milk, cheese, and infant formula. L. monocytogenes ATCC 19115 in the samples was then enumerated on Oxford agar, and DNA was extracted from the samples before and after incubation at 36 degrees C for 4 h. A set of primers and hybridization probe designed in this study was then used to detect the pathogen. The standard curve was then prepared by plotting cycle threshold values for each dilution versus L. monocyto genes cell counts (log CPU). The specificity of the set of primers and hybridization probe was appropriate. A 4-h incubation at 36 degrees C before DNA extraction produced optimum standard curves in comparison to the results for a 0-h incubation. Thus, a 4-h incubation at 36 degrees C was applied for monitoring L. monocytogenes in collected food samples. To monitor L. monocyto genes in foods, 533 samples (ham, 129; sausage, 226; ground meat, 72; processed cheese, 54; processed milk, 42; and infant formula, 10) were collected from retail markets and from the step before pasteurization in plants. Of all 533 samples, 4 samples (0.8%) showed positive signals in RT-PCR. Two samples from hams (1.6%) and two samples from sausages (0.9%) were determined to be positive for L. monocytogenes at <100 CFU/g. The results indicate that the RT-PCR detection method with the set of primers and hybridization probe designed in this study should be useful in monitoring for L. monocytogenes in processed meat and milk products.
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