Eicosapentaenoic Acid and Docosahexaenoic Acid Suppress Th2 Cytokine Expression in RBL-2H3 Basophilic Leukemia Cells
- Authors
- Jin, Mirim; Park, Sunyoung; Park, Bo-Kyung; Choi, Jeong June; Yoon, Soo Jeong; Yang, Mihi; Pyo, Myoung Yun
- Issue Date
- Feb-2014
- Publisher
- MARY ANN LIEBERT, INC
- Keywords
- docosahexaenoic acid; eicosapentaenoic acid; IL-4; IL-13; RBL-2H3 basophilic leukemia cells
- Citation
- JOURNAL OF MEDICINAL FOOD, v.17, no.2, pp 198 - 205
- Pages
- 8
- Journal Title
- JOURNAL OF MEDICINAL FOOD
- Volume
- 17
- Number
- 2
- Start Page
- 198
- End Page
- 205
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/10998
- DOI
- 10.1089/jmf.2013.2935
- ISSN
- 1096-620X
1557-7600
- Abstract
- It is known that the intake of omega-3 fatty acids, such as eicosapentaenoic (EPA) and docosahexaenoic acid (DHA), is beneficial for preventing and/or treating allergic diseases. The pathogenesis of allergic diseases is associated with overactivation of Th2-skewed immunity. Basophils generate large amounts of Th2 cytokines such as interleukin (IL)-4 and IL-13, which are critically involved in allergic inflammation. We investigated how EPA and DHA affect Th2 cytokine expression in phorbol 12-myristate 13-acetate- and ionomycin (PI)-activated RBL-2H3 basophilic leukemia cells. EPA and DHA induced a dramatic decrease in the production of IL-4 and IL-13 and their transcription in a dose-dependent manner. Luciferase assays of RBL-2H3 cells stably expressing Il4 and Il13 promoter-reporter plasmids demonstrated a significant suppression of PI-induced promoter activation. Analysis of certain transcription factors revealed that nuclear expression of c-Fos and the mRNA expression were suppressed by EPA and DHA. Furthermore, they significantly inhibited the nuclear expression and translocation of nuclear factor of activated T cells (NF-AT)1. In contrast, the expression levels of nuclear factor kappa-B (NF-B), GATA-binding proteins (GATAs), and CCAAT/enhancer binding protein alpha (C/EBP) were not significantly affected by EPA and DHA. Phosphorylation of extracellular signal-related kinase was inhibited by EPA and DHA, and phosphorylation of p38 mitogen-activated protein kinase was decreased by DHA, but not by EPA. Taken together, our data suggest that EPA and DHA may suppress Th2-skewed allergic immune responses by inhibiting the expression of basophilic IL-4 and IL-13.
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