Interleukin-32 delta interacts with IL-32 beta and inhibits IL-32 beta-mediated IL-10 production
- Authors
- Kang, Jeong-Woo; Park, Yun Sun; Lee, Dong Hun; Kim, Man Sub; Bak, Yesol; Park, Su Ho; Ham, Sun Young; Yang, Young; Hong, Jin Tae; Yoon, Do-Young
- Issue Date
- Nov-2013
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- IL-32 beta; IL-32 delta; IL-10; PKC delta; U937; Isoform interaction
- Citation
- FEBS LETTERS, v.587, no.23, pp 3776 - 3781
- Pages
- 6
- Journal Title
- FEBS LETTERS
- Volume
- 587
- Number
- 23
- Start Page
- 3776
- End Page
- 3781
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/11178
- DOI
- 10.1016/j.febslet.2013.10.019
- ISSN
- 0014-5793
1873-3468
- Abstract
- There is growing evidence for multifunctional properties of IL-32. We previously demonstrated that IL-32 beta upregulates IL-10 production through the association with PKC delta. In this study, we examined the effects of other IL-32 isoforms on IL-10 production. We found that IL-32 delta decreased IL-10 production and investigated the inhibitory mechanism of IL-32 delta. We showed that IL-32 delta suppressed IL-32 beta binding to PKC delta by interacting with IL-32 beta. The inhibitory effect of IL-32 delta on IL-32 beta association with PKC delta was further verified by immuno-fluorescence staining. The co-localization of IL-32 beta and PKC delta around the nuclear membrane was disrupted by IL-32 delta. Our data therefore indicate that IL-32 delta plays an inhibitory role against IL-32 beta function, which also suggests that IL-32 may be regulated by its own isoform. Structured summary of protein interactions: PKC delta physically interacts with IL-32 beta by anti bait coimmunoprecipitation (View interaction) IL-32 beta physically interacts with PKC delta by anti tag coimmunoprecipitation (View interaction) IL-32 beta physically interacts with IL-32 delta by anti tag coimmunoprecipitation (1, 2) PKC delta and IL-32 beta colocalize by fluorescence microscopy (view interaction) (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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