EphrinA5-EphA7 complex induces apoptotic cell death via TNFR1
- Authors
- Lee, Haeryung; Park, Eunjeong; Kim, Yujin; Park, Soochul
- Issue Date
- May-2013
- Publisher
- KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
- Keywords
- apoptosis; ephrinA5; EphA7; TNFR1
- Citation
- MOLECULES AND CELLS, v.35, no.5, pp 450 - 455
- Pages
- 6
- Journal Title
- MOLECULES AND CELLS
- Volume
- 35
- Number
- 5
- Start Page
- 450
- End Page
- 455
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/11295
- DOI
- 10.1007/s10059-013-0072-3
- ISSN
- 1016-8478
0219-1032
- Abstract
- A previous study showed that the EphA7 receptor regulates apoptotic cell death during early brain development. In this study, we provide evidence that the EphA7 receptor interacts with death receptors such as tumor necrosis factor receptor 1 (TNFR1) to decrease cell viability. We showed that ephrinA5 stimulates EphA7 to activate the TNFR1-mediated apoptotic signaling pathway. In addition, a pull-down assay using biotinylated ephrinA5-Fc revealed that ephrinA5-EphA7 complexes recruit TNFR1 to form a multi-protein complex. Immunocytochemical staining analysis showed that EphA7 was co-localized with TNFR1 on the cell surface when cells were incubated with ephrinA5 at low temperatures. Finally, both the internalization motif and death domain of TNFR1 was important for interacting with an intracytoplasmic region of EphA7; this interaction was essential for inducing the apoptotic signaling cascade. This result suggests that a distinct multi-protein complex comprising ephrinA5, EphA7, and TNFR1 may constitute a platform for inducing caspase-dependent apoptotic cell death.
- Files in This Item
-
Go to Link
- Appears in
Collections - 이과대학 > 생명시스템학부 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.