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RNA interference-mediated repression of S6 kinase 1 impairs root nodule development in soybean

Authors
Um, Ji-HyunKim, SunghanKim, Yun-KyoungSong, Seok-BoLee, Suk-HaVerma, Desh Pal S.Cheon, Choong-Ill
Issue Date
Mar-2013
Publisher
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
Keywords
kinase; nodulation; RNAi; TOR
Citation
MOLECULES AND CELLS, v.35, no.3, pp 243 - 248
Pages
6
Journal Title
MOLECULES AND CELLS
Volume
35
Number
3
Start Page
243
End Page
248
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/11328
DOI
10.1007/s10059-013-2315-8
ISSN
1016-8478
0219-1032
Abstract
Symbiotic nodule formation on legume roots is characterized with a series of developmental reprograming in root tissues, including extensive proliferation of cortical cells. We examined a possible involvement of the target of rapamycin (TOR) pathway, a central regulator of cell growth and proliferation in animals and yeasts, during soybean nodule development. Our results show that transcription of both GmTOR and its key downstream effector, GmS6K1, are activated during nodulation, which is paralleled with higher kinase activities of these gene products as well. RNAi-mediated knockdown of GmS6K1 impaired the nodule development with severely reduced nodule weight and numbers. In addition, expression of a few nodulins including leghemoglobin was also decreased, and consequently nitrogen fixation was found to be reduced by half. Proteomic analysis of the GmS6K1-RNAi nodules identified glutamine synthetase (GS), an essential enzyme for nitrogen assimilation in nodules, as one of the proteins that are significantly down regulated. These results appear to provide solid evidence for a functional link between GmS6K1 and nodule development.
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