A Gene Trap Knockout of the Tiam-1 Protein Results in Malformation of the Early Embryonic Brain
- Authors
- Yoo, Sooyeon; Kim, Yujin; Lee, Haeryung; Park, Sungjeong; Park, Soochul
- Issue Date
- Jul-2012
- Publisher
- KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
- Keywords
- early brain development; Rac; Tiam-1
- Citation
- MOLECULES AND CELLS, v.34, no.1, pp 103 - 108
- Pages
- 6
- Journal Title
- MOLECULES AND CELLS
- Volume
- 34
- Number
- 1
- Start Page
- 103
- End Page
- 108
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/11876
- DOI
- 10.1007/s10059-012-0119-x
- ISSN
- 1016-8478
0219-1032
- Abstract
- Tiam-1 has been implicated in the development of the central nervous system. However, the in vivo function of Tiam-1 has not been fully determined in the developing mouse brain. In this study, we generated Tiam-1 knockout mice using a Tiam-1 gene-trapped embryonic stem cell line. Insertion of a gene trap vector into a genomic site downstream of exon 5 resulted in a mutant allele encoding a truncated protein fused with the beta-geo LacZ gene. Primary mouse embryonic fibroblasts lacking Tiam-1 revealed a significant decrease in Rac activity and cell proliferation. In addition, whole-mount embryonic LacZ expression analysis demonstrated that Tiam-1 is specifically expressed in regions of the developing brain, such as the caudal telencephalon and rostral diencephalon. More importantly, mouse embryos deficient in Tiam-1 gene expression displayed a severe defect in embryonic brain development, including neural tube closure defects or a dramatic decrease in brain size. These findings suggest that embryonic Tiam-1 expression plays a critical role during early brain development in mice.
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