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Mxi1 influences cyst formation in three-dimensional cell culture

Authors
Yook, Yeon JooYoo, Kyung HyunSong, Seon AhSeo, Min JiKo, Je YeongKim, Bo HyeLee, Eun JiChang, EunSunWoo, Yu MiPark, Jong Hoon
Issue Date
Mar-2012
Publisher
KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
Keywords
Cyst formation; mIMCD-3 cell; Mxi1; 3-D culture
Citation
BMB REPORTS, v.45, no.3, pp 189 - 193
Pages
5
Journal Title
BMB REPORTS
Volume
45
Number
3
Start Page
189
End Page
193
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/11957
DOI
10.5483/BMBRep.2012.45.3.189
ISSN
1976-6696
1976-670X
Abstract
Cyst formation is a major characteristic of ADPKD and is caused by the abnormal proliferation of epithelial cells. Renal cyst formation disrupts renal function and induces diverse complications. The mechanism of cyst formation is unclear. mIMCD-3 cells were established to develop simple epithelial cell cysts in 3-D culture. We confirmed previously that Mxi1 plays a role in cyst formation in Mxi1-deficient mice. Cysts in Mxi1 transfectanted cells were showed by collagen or mebiol gels in 3-D cell culture system. Causative genes of ADPKD were measured by q RT-PCR. Herein, Mxi1 transfectants rarely formed a simple epithelial cyst and induced cell death. Overexpression of Mxi1 resulted in a decrease in the PKD1, PKD2 and c-myc mRNA relating to the pathway of cyst formation. These data indicate that Mxi1 influences cyst formation of mIMCD-3 cells in 3-D culture and that Mxi1 may control the mechanism of renal cyst formation. [BMB reports 2012; 45(3): 189-193]
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