L-Kynurenine-induced apoptosis in human NK cells is mediated by reactive oxygen species
- Authors
- Song, Hyunkeun; Park, Hyunjin; Kim, Yeong-Seok; Kim, Kwang Dong; Lee, Hyun-Kyung; Cho, Dae-Ho; Yang, Jae-Wook; Hur, Dae Young
- Issue Date
- Aug-2011
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- NK cell; ROS; IDO; L-Kynurenine; Apoptosis
- Citation
- INTERNATIONAL IMMUNOPHARMACOLOGY, v.11, no.8, pp 932 - 938
- Pages
- 7
- Journal Title
- INTERNATIONAL IMMUNOPHARMACOLOGY
- Volume
- 11
- Number
- 8
- Start Page
- 932
- End Page
- 938
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/12530
- DOI
- 10.1016/j.intimp.2011.02.005
- ISSN
- 1567-5769
1878-1705
- Abstract
- Recent studies have shown that indoleamine 2,3-dioxygenase (IDO) plays a pivotal role in the modulation of immune response against tumor and virus infection. Here we demonstrate the pro-apoptotic effect of L-kynurenine, a tryptophan catabolite of IDO, on human NK cell line, NK92 MI. Treatment with L-kynurenine dose-dependently induced growth inhibition and apoptosis in NK92 MI cells. Treatment with the antioxidant NAC completely protected cells from L-kynurenine-induced apoptosis. Moreover, we found that treatment with Z-VAD-fmk and ZB4 slightly inhibited L-kynurenine-induced apoptosis, suggesting that L-kynurenine-induced apoptosis in NK cells occurs primarily through an ROS mediated pathway. We observed that the presence of NAC blocks cytochrome c release and activation of caspase-3 during L-kynurenine-induced apoptosis. Overall, we conclude that L-kynurenine resulting from 100 can cause cell death via ROS pathway in NK cells. Our findings provide a new insight into the interaction between NK cells and IDO positive cancer cells in regulating immune responses. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.
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