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Inhibitory effects of OD 78 [3-(4-bromo-phenoxy)-4,5-dihydroxybenzoic acid-methyl ester] on the proliferation and migration of TNF-alpha-induced rat aortic smooth muscle cells

Authors
Lim, YongTudev, MunkhtsetsegPark, Eun-SeokKim, Won-ShikLim, Il-HoLee, Mi-YeaLee, HeesoonJung, Jae-KyungHong, Jin-TaeYoo, Hwan-SooLee, Myung-KooPyo, Myoung-YunYun, Yeo-Pyo
Issue Date
Jul-2011
Publisher
PHARMACEUTICAL SOC KOREA
Keywords
Rat aortic smooth muscle cell; TNF-alpha; Migration; p38; MMP-9
Citation
ARCHIVES OF PHARMACAL RESEARCH, v.34, no.7, pp 1191 - 1199
Pages
9
Journal Title
ARCHIVES OF PHARMACAL RESEARCH
Volume
34
Number
7
Start Page
1191
End Page
1199
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/12550
DOI
10.1007/s12272-011-0718-7
ISSN
0253-6269
1976-3786
Abstract
The proliferation and migration of vascular smooth muscle cells (VSMCs) play important roles in the formation and progression of intimal thickening in early-phase atherosclerosis and in restenosis after vascular injury. Tumor necrosis factor-alpha (TNF-alpha) is released from macrophages in atherosclerotic lesions and from neointimal vascular smooth muscle cells after balloon-injury. Obovatol, a major biphenolic component isolated from the Magnolia obovata leaf, is known to have anti-inflammatory and antitumor activities. The goal of this study was to examine the cardioprotective effects of the obovatol derivative OD 78 on the TNF-alpha-induced proliferation and migration of rat aortic smooth muscle cells (RASMCs). The antiproliferative effects of OD 78 on RASMCs were examined by cell counting and [(3)H]-thymidine incorporation assays. Treatment of cells with 1-4 mu M OD 78 inhibited the proliferation and DNA synthesis of TNF-alpha-stimulated RASMCs in a concentration-dependent manner, without cytotoxicity. Treatment with OD 78 inhibited TNF-alpha-mediated p38 phosphorylation, but did not change the activation of extracellular signal-regulated kinase or c-Jun N-terminal kinase. Furthermore, treatment with OD 78 decreased TNF-alpha-induced levels of cyclin E, cyclin D1, CDK2, proliferating cell nuclear antigen, and phosphorylated retinoblastoma protein, but not the CDK4 expression level. Also, OD 78 inhibits the migration of TNF-alpha-induced RASMC in transwells. OD 78 treatment strongly decreased matrix metalloproteinase-9 (MMP-9) expression in a dose-dependent manner, but the MMP-2 expression was unchanged. These results show that OD 78 may be developed as a potential antiproliferative agent for the treatment of angioplasty restenosis and atherosclerosis.
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