beta-Glycerophosphate accelerates RANKL-induced osteoclast formation in the presence of ascorbic acid
- Authors
- Noh, A. Long Sae Mi; Yim, Mijung
- Issue Date
- Mar-2011
- Publisher
- GOVI-VERLAG PHARMAZEUTISCHER VERLAG GMBH
- Citation
- PHARMAZIE, v.66, no.3, pp 195 - 200
- Pages
- 6
- Journal Title
- PHARMAZIE
- Volume
- 66
- Number
- 3
- Start Page
- 195
- End Page
- 200
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/12638
- DOI
- 10.1691/ph.2011.0779
- ISSN
- 0031-7144
- Abstract
- Despite numerous reports of the synergistic effects of beta-glycerophosphate and ascorbic acid in inducing the differentiation of osteoblasts, little is known about their roles in osteoclastic differentiation. Therefore, we investigated the effect of beta-glycerophosphate on osteoclastogenesis in the presence of ascorbic acid using primary mouse bone marrow cultures treated with macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor (NF)-kappa B ligand (RANKL). p-Glycerophosphate dose-dependently increased RANKL-induced osteoclast formation in the presence of ascorbic acid. This stimulatory effect was apparent when beta-glycerophosphate and ascorbic acid were only added during the late stages of the culture period, indicating that they influence later events in osteoclastic differentiation. While the combination of beta-glycerophosphate and ascorbic acid inhibited RANKL-stimulated activation of ERK and p38, and degradation of I kappa B, it increased the induction of c-Fos and NFATc1. In addition, beta-glycerophosphate and ascorbic acid together enhanced the induction of COX-2 following RANKL stimulation. Taken together, our data suggest that beta-glycerophosphate and ascorbic acid have synergistic effects on osteoclast formation, increasing RANKL-mediated induction of c-Fos, NFATc1 and COX-2 in osteoclast precursors.
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