Combination of Enrichment and PCR in Rapid Semi-Quantification of Bacillus cereus in Fresh-Cut Vegetables
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최유경 | - |
dc.contributor.author | Sujung Lee | - |
dc.contributor.author | 윤요한 | - |
dc.date.available | 2021-02-22T05:23:10Z | - |
dc.date.issued | 2020-08 | - |
dc.identifier.issn | 1229-1153 | - |
dc.identifier.issn | 2465-9223 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/1263 | - |
dc.description.abstract | Widespread consumption of fresh-cut vegetables without cooking results in ingestion of major foodborne pathogens including Bacillus cereus. In this study, we aimed to develop a method to rapidly detect B. cereus in fresh-cut vegetables by combining commercial PCR analysis with enrichment of the pathogenic levels. A mixture of B. cereus strains (KCTC1013, KCTC1014, KCTC1092, KCTC1094, and KCTC3624) was inoculated on the surface of fresh-cut cabbage lettuce (20 g) and baby leafy vegetables (10 g) to concentration 1, 2, 3, 4, and 5 log CFU/g. Eighty milliliters of TSB with 0.15% polymyxin B was used for cabbage lettuce, and 90 mL of medium was used for baby leafy vegetables and incubated at 42 o C for 0, 2, 3, 4, 5, 6, and 7 h. One milliliter of the enriched media was plated on mannitol-egg yolk-polymyxin agar for quantification, and another 1 mL was used for DNA extraction for PCR analysis. Additionally, the minimum number of sub-samples to be tested from a pack of fresh-cut vegetable samples was determined using 5 sub-samples. The results from this study showed that for detecting B. cereus in fresh-cut cabbage lettuce, 3, 4, 5, 6, and 7 h enrichment were required to at least detect 5, 4, 3, 2, and 1 log CFU/g of B. cereus, respectively. B. cereus in fresh-cut baby leafy vegetables could be detected after 2, 3, 4, 5, and 6 h of enrichment at 5, 4, 3, 2, and 1 log CFU/g, respectively, using a combination of enrichment and PCR analysis. To determine if a pack of fresh-cut vegetable is positive, the minimum number of sub-samples should be 3. These results can be used to develop a rapid detection method to semi-quantify B. cereus in fresh-cut vegetable samples combining enrichment and PCR. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 한국식품위생안전성학회 | - |
dc.title | Combination of Enrichment and PCR in Rapid Semi-Quantification of Bacillus cereus in Fresh-Cut Vegetables | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.13103/JFHS.2020.35.4.319 | - |
dc.identifier.bibliographicCitation | 한국식품위생안전성학회지, v.35, no.4, pp 319 - 325 | - |
dc.citation.title | 한국식품위생안전성학회지 | - |
dc.citation.volume | 35 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 319 | - |
dc.citation.endPage | 325 | - |
dc.identifier.kciid | ART002616352 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordAuthor | Bacillus cereus | - |
dc.subject.keywordAuthor | Fresh-cut vegetables | - |
dc.subject.keywordAuthor | Rapid detection | - |
dc.subject.keywordAuthor | Enrichment | - |
dc.subject.keywordAuthor | PCR | - |
dc.identifier.url | http://foodsafety.or.kr/journal/article.php?code=75437 | - |
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