Detailed Information

Cited 0 time in webofscience Cited 81 time in scopus
Metadata Downloads

Pegylated poly-L-arginine derivatives of chitosan for effective delivery of siRNA

Authors
Noh, Sang MyoungPark, Myung OkShim, GayongHan, Su EunLee, Han YoungHuh, Jun HyukKim, Myoung SukChoi, Jin JooKim, KwangmeyungKwon, Ick ChanKim, Jin-SeokBaek, Kwang-HyunOh, Yu-Kyoung
Issue Date
14-Jul-2010
Publisher
ELSEVIER SCIENCE BV
Keywords
Chitosan; Polyarginine; Polyethylene glycol; siRNA; Serum stability
Citation
JOURNAL OF CONTROLLED RELEASE, v.145, no.2, pp.159 - 164
Journal Title
JOURNAL OF CONTROLLED RELEASE
Volume
145
Number
2
Start Page
159
End Page
164
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/13169
DOI
10.1016/j.jconrel.2010.04.005
ISSN
0168-3659
Abstract
For delivery of siRNA, chitosan (CS) was derivatized with poly-L-arginine (PLR) and polyethylene glycol (PEG). The formation of polyplexes with siRNA was confirmed by gel retardation. The PLR-grafted CS formed nanosized particles with siRNA. PLR-grafted CS showed higher cellular delivery efficiency of siRNA than did CS. pegylated CS, PLR, or pegylated PLR. The extent of reduction in the expression of fluorescent proteins was highest following treatment of the cells using PLR derivatives of CS in complexes with specific siRNAs. Cell viability was greater in populations treated with pegylated CS-PLR than in those treated with PLR. Hemolysis of erythrocytes was reduced upon conjugation of PLR with CS. The delivery of siRNAs via pegylated CS-PLR revealed little dependence on serum. Molecular imaging techniques revealed that the intratumoral administration of red fluorescent protein-specific siRNA in complexes with pegylated CS-PLR significantly silenced the expression of red fluorescent proteins in tumor tissues in vivo. These results indicate that pegylated CS-PLR might be useful for in vivo delivery of therapeutic siRNAs. (C) 2010 Elsevier B.V. All rights reserved.
Files in This Item
There are no files associated with this item.
Appears in
Collections
약학대학 > 약학부 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Kim, Jin Seok photo

Kim, Jin Seok
약학대학 (약학부)
Read more

Altmetrics

Total Views & Downloads

BROWSE