Detailed Information

Cited 0 time in webofscience Cited 8 time in scopus
Metadata Downloads

Identification of uricase as a potential target of plant thioredoxin: Implication in the regulation of nodule development

Authors
Du, HuiKim, SunghanNam, Kyoung HeeLee, Myung-SokSon, OraLee, Suk-HaCheon, Choong-Ill
Issue Date
Jun-2010
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Keywords
Thioredoxin; Nodulin-35; Uricase; Nodule development
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.397, no.1, pp.22 - 26
Journal Title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume
397
Number
1
Start Page
22
End Page
26
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/13185
DOI
10.1016/j.bbrc.2010.05.040
ISSN
0006-291X
Abstract
During symbiotic nodule development in legume roots, early signaling events between host and rhizobia serve critical determinants for the proper onset of nodule morphogenesis, nitrogen fixation, and assimilation. Previously we isolated thioredoxin from soybean nodules as one of differentially expressed genes during nodulation and noted its positive role in nitrogen fixation. To identify the target proteins of thioredoxin in nodules, we used thioredoxin affinity chromatography followed by mass spectrometry. Nodulin-35, a subunit of uricase, was found to be a target of thioredoxin. Their interaction was confirmed by pull-down assay and by bimolecular fluorescent complementation. With an increased uricase activity observed also in the presence of thioredoxin, these results appear to implicate a novel role of thioredoxin in the regulation of enzyme activities involved in nodule development and nitrogen fixation. (C) 2010 Elsevier Inc. All rights reserved.
Files in This Item
Go to Link
Appears in
Collections
이과대학 > 생명시스템학부 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Cheon, Choong Ill photo

Cheon, Choong Ill
이과대학 (생명시스템학부)
Read more

Altmetrics

Total Views & Downloads

BROWSE