EphA8-ephrinA5 Signaling and Clathrin-Mediated Endocytosis Is Regulated by Tiam-1, a Rac-Specific Guanine Nucleotide Exchange Factor
- Authors
- Yoo, Sooyeon; Shin, Jongdae; Park, Soochul
- Issue Date
- Jun-2010
- Publisher
- KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
- Keywords
- endocytosis; EphA8; ephrin-A5
- Citation
- MOLECULES AND CELLS, v.29, no.6, pp 603 - 609
- Pages
- 7
- Journal Title
- MOLECULES AND CELLS
- Volume
- 29
- Number
- 6
- Start Page
- 603
- End Page
- 609
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/13200
- DOI
- 10.1007/s10059-010-0075-2
- ISSN
- 1016-8478
0219-1032
- Abstract
- Recent studies indicate that endocytosis of Eph-ephrin complexes may be one of the mechanisms by which a high affinity cell-cell adhesion is converted to a repulsive interaction. In this study, we show that EphA8 undergoes clathrin-mediated endocytosis upon treatment with ephrin-A5, and that EphA8 is associated tightly with Tiam-1, a Rac-specific guanine nucleotide exchange factor. Analysis of EphA8 deletion mutants revealed that a juxtamembrane region in EphA8 is critically involved in endocytosis of EphA8-ephrinA5 complexes. An EphA8 mutant lacking this juxtamembrane portion was defective for endocytosis with ephrinA5, and also displayed a weak association with Tiam-1. Expression of an endocytosis-defective version of EphA8 resulted in a low level of Rac activity in response to ephrin-A5 stimulation. More importantly, down-regulation of Tiam-1 resulted in inefficient endocytosis of EphA8-ephrinA5 complexes. These results suggest that Tiam-1 plays a role in clathrin-dependent endocytosis of EphA8-ephrinA5 complexes.
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