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Expression of the atf1(+) gene is upregulated in fission yeast under nitrosative and nutritional stresses

Authors
Song, S. -H.Kim, B. -M.Lim, C. -J.Song, Y. S.Park, E. -H.
Issue Date
Nov-2009
Publisher
CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS
Keywords
Atf1; nitric oxide; nitrogen starvation; Schizosaccharomyces pombe
Citation
CANADIAN JOURNAL OF MICROBIOLOGY, v.55, no.11, pp.1323 - 1327
Journal Title
CANADIAN JOURNAL OF MICROBIOLOGY
Volume
55
Number
11
Start Page
1323
End Page
1327
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/13658
DOI
10.1139/W09-087
ISSN
0008-4166
Abstract
This work was designed to assess regulation of the atf1+ gene in the fission yeast Schizosaccharomyces pombe under nitrosative and nutritional stresses, using the atf1+-lacZ fusion gene and RT-PCR. Nitric oxide (NO)-generating sodium nitroprusside (SNP; 10 mu mol/L) and nitrogen depletion significantly enhanced synthesis of beta-galactosidase from the atf1+-lacZ fusion gene in S. pombe Pap1-positive KP1 cells, but not in S. pombe Pap1-negative TP108-3C cells. SNP (10 mu mol/L) and nitrogen depletion also caused a significant increase in atf1+ mRNA levels in Pap1-positive cells, but not in Pap1-negative cells. Depletion of glucose marginally increased synthesis of beta-galactosidase from the fusion gene in S. pombe Pap1-positive cells. Taken together, the S. pombe atf1+ gene is upregulated by nitrosative and nutritional stresses on a transcriptional level, possibly via the mediation of Pap1.
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