Changes in Cytochrome c Oxidase and NO in Rat Lung Mitochondria Following Iron Overload
- Authors
- Kim, Misun; Hong, Min-a; Song, Eunsook
- Issue Date
- 30-Jun-2009
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- lung mitochondria; iron overload; cytochrome c oxidase subunit IV; nitric oxide; nitric oxide synthase
- Citation
- ANIMAL CELLS AND SYSTEMS, v.13, no.2, pp 105 - 112
- Pages
- 8
- Journal Title
- ANIMAL CELLS AND SYSTEMS
- Volume
- 13
- Number
- 2
- Start Page
- 105
- End Page
- 112
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/13733
- DOI
- 10.1080/19768354.2009.9647200
- ISSN
- 1976-8354
2151-2485
- Abstract
- In this study, the effects of iron on cytochrome c oxidase (CcO) in rat lung mitochondria were examined. Similar to liver mitochondria, iron accumulated considerably in lung mitochondria (more than 2-fold). Likewise, the reactive oxygen species and nitric oxide (NO) content of mitochondria were increased by more than 50% and 100%, respectively. NO might be produced by nitric oxide synthase (NOS), eNOS and NOS type, with particular contribution by NOS in mitochondria. The respiratory control ratio of iron-overloaded lung mitochondria dropped to nearly 50% due to increased state 4. Likewise, cytochrome c oxidase activity was lowered significantly to approximately 50% due to excess iron. Real-time PCR revealed that the expression of isoforms 1 and 2 of subunit IV of CcO was enhanced greatly under excess iron conditions. Taken together, these results show that oxidative phosphorylation within lung mitochondria may be influenced by iron overload through changes in cytochrome c oxidase and NO.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 이과대학 > 생명시스템학부 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.