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Changes in Cytochrome c Oxidase and NO in Rat Lung Mitochondria Following Iron Overload

Authors
Kim, MisunHong, Min-aSong, Eunsook
Issue Date
30-Jun-2009
Publisher
TAYLOR & FRANCIS LTD
Keywords
lung mitochondria; iron overload; cytochrome c oxidase subunit IV; nitric oxide; nitric oxide synthase
Citation
ANIMAL CELLS AND SYSTEMS, v.13, no.2, pp.105 - 112
Journal Title
ANIMAL CELLS AND SYSTEMS
Volume
13
Number
2
Start Page
105
End Page
112
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/13733
DOI
10.1080/19768354.2009.9647200
ISSN
1976-8354
Abstract
In this study, the effects of iron on cytochrome c oxidase (CcO) in rat lung mitochondria were examined. Similar to liver mitochondria, iron accumulated considerably in lung mitochondria (more than 2-fold). Likewise, the reactive oxygen species and nitric oxide (NO) content of mitochondria were increased by more than 50% and 100%, respectively. NO might be produced by nitric oxide synthase (NOS), eNOS and NOS type, with particular contribution by NOS in mitochondria. The respiratory control ratio of iron-overloaded lung mitochondria dropped to nearly 50% due to increased state 4. Likewise, cytochrome c oxidase activity was lowered significantly to approximately 50% due to excess iron. Real-time PCR revealed that the expression of isoforms 1 and 2 of subunit IV of CcO was enhanced greatly under excess iron conditions. Taken together, these results show that oxidative phosphorylation within lung mitochondria may be influenced by iron overload through changes in cytochrome c oxidase and NO.
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