The Change of Taurine Transport in Variable Stress States through the Inner Blood-Retinal Barrier using In Vitro Model
- Authors
- Kang, Young-Sook; Lee, Na-Young; Chung, Yeon-Yee
- Issue Date
- Apr-2009
- Publisher
- KOREAN SOC APPLIED PHARMACOLOGY
- Keywords
- Taurine transport; Inner blood-retinal barrier; Conditionally immortalized rat retinal capillary endothelial cells; Oxidative stress; Hyperglycemic condition; Extracellular calcium
- Citation
- BIOMOLECULES & THERAPEUTICS, v.17, no.2, pp 175 - 180
- Pages
- 6
- Journal Title
- BIOMOLECULES & THERAPEUTICS
- Volume
- 17
- Number
- 2
- Start Page
- 175
- End Page
- 180
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/13771
- DOI
- 10.4062/biomolther.2009.17.2.175
- ISSN
- 1976-9148
2005-4483
- Abstract
- Taurine is the most abundant free amino acid in the retina and transported into retina via taurine transporter (TauT) at the inner blood-retinal barrier (iBRB). In the present study, we investigated whether the taurine transport at the iBRB is regulated by oxidative stress or disease-like state in a conditionally immortalized rat retinal capillary endothelial cell line (TR-iBRB) used as an in vitro model of iBRB. First, [H-3]taurine uptake and efflux by TR-iBRB were regulated in the presence of extracellular Ca2+. [H-3]Taurine uptake was inhibited and efflux was enhanced under Ca2+ free condition in the cells. In addition, oxidative stress inducing agents such as tumor necrosis factor-alpha (TNF-alpha), lipopolysaccharide (LPS), diethyl maleate (DEM) and glutamate increased [H-3]taurine uptake and decreased [H-3]taurine efflux in TR-iBRB cells. Whereas, 3-morpholinosydnonimine (SIN-1), which is known to NO donor decreased [H-3]taurine uptake. Lastly, TR-iBRB cells exposed to high glucose (25 mM) medium and the [H-3]taurine uptake was reduced about 20% at the condition. Also, [H-3]taurine uptake was decreased by cytochalasin B, which is known to glucose transport inhibitor. In conclusion, taurine transport in TR-iBRB cells is regulated diversely at extracellular Ca2+, oxidative stress and hyperglycemic condition. It suggested that taurine would play a role as a retinal protector in diverse disease states.
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