NDRG2 expression decreases with tumor stages and regulates TCF/beta-catenin signaling in human colon carcinoma
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Young-Jun | - |
dc.contributor.author | Yoon, Sun Y. | - |
dc.contributor.author | Kim, Jong-Tae | - |
dc.contributor.author | Song, Eun Y. | - |
dc.contributor.author | Lee, Hee G. | - |
dc.contributor.author | Son, Hyun J. | - |
dc.contributor.author | Kim, Soo Y. | - |
dc.contributor.author | Cho, Daeho | - |
dc.contributor.author | Choi, Inpyo | - |
dc.contributor.author | Kim, Joo H. | - |
dc.contributor.author | Kim, Jae W. | - |
dc.date.available | 2021-02-22T14:17:42Z | - |
dc.date.issued | 2009-04 | - |
dc.identifier.issn | 0143-3334 | - |
dc.identifier.issn | 1460-2180 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/13791 | - |
dc.description.abstract | NDRG (N-Myc downstream-regulated gene)-2 is a member of the NDRG family. Although it has been suggested that NDRG2 is involved in cellular differentiation and tumor suppression, its intracellular signal and regulatory mechanism are not well known. Here, we show the differential expression of NDRG2 in human colon carcinoma cell lines and tissues by reverse transcription-polymerase chain reaction and immunohistochemical analyses with monoclonal antibody against NDRG2. NDRG2 was strongly expressed in normal colonic mucosa and colonic adenomatous tissues (25 of 25) but not in all invasive cancer tissues [44 of 99 (44%)]. Most distinctive results indicated that the high expression level of NDRG2 has a positive correlation with tumor differentiation and inverse correlation with tumor invasion depth and Dukes' stage of colon adenocarcinoma. To investigate the roles of NDRG2 in tumorigenesis, we used in vitro cell culture system. SW620 colon cancer cell line with a low level of intrinsic NDRG2 protein was transfected with NDRG2-expressing plasmid. TOPflash luciferase reporter assay showed that the transcriptional activity of T-cell factor (TCF)/lymphoid enhancer factor (LEF) was reduced by NDRG2 introduction, but not by the introduction of mutant NDRG2 generated by deletion or site-directed mutagenesis. Intracellular beta-catenin levels were slightly reduced in the NDRG2-transfected SW620 cells and this regulation of beta-catenin stability and TCF/LEF activity were mediated through the modulation of glycogen synthase kinase-3beta activity by NDRG2 function. Our results suggest that NDRG2 might play a pivotal role as a potent tumor suppressor by the attenuation of TCF/beta-catenin signaling for the maintenance of healthy colon tissues. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.title | NDRG2 expression decreases with tumor stages and regulates TCF/beta-catenin signaling in human colon carcinoma | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1093/carcin/bgp047 | - |
dc.identifier.scopusid | 2-s2.0-64349090438 | - |
dc.identifier.wosid | 000264889100007 | - |
dc.identifier.bibliographicCitation | CARCINOGENESIS, v.30, no.4, pp 598 - 605 | - |
dc.citation.title | CARCINOGENESIS | - |
dc.citation.volume | 30 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 598 | - |
dc.citation.endPage | 605 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | GLYCOGEN-SYNTHASE KINASE-3 | - |
dc.subject.keywordPlus | BETA-CATENIN | - |
dc.subject.keywordPlus | CELL DIFFERENTIATION | - |
dc.subject.keywordPlus | SUPPRESSOR GENE | - |
dc.subject.keywordPlus | DOWN-REGULATION | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | MYC | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | APC | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Sookmyung Women's University. Cheongpa-ro 47-gil 100 (Cheongpa-dong 2ga), Yongsan-gu, Seoul, 04310, Korea02-710-9127
Copyright©Sookmyung Women's University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.