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Characterization of the mechanism of zidovudine uptake by rat conditionally immortalized syncytiotrophoblast cell line TR-TBT

Authors
Sai, Y.Nishimura, T.Shimpo, S.Chishu, T.Sato, K.Kose, N.Terasaki, T.Mukai, C.Kitagaki, S.Miyakoshi, N.Kang, Y. -S.Nakashima, E.
Issue Date
Jul-2008
Publisher
SPRINGER/PLENUM PUBLISHERS
Keywords
AZT; blood-placenta barrier; syncytiotrophoblast; transporter; TR-TBT
Citation
PHARMACEUTICAL RESEARCH, v.25, no.7, pp.1647 - 1653
Journal Title
PHARMACEUTICAL RESEARCH
Volume
25
Number
7
Start Page
1647
End Page
1653
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/14227
DOI
10.1007/s11095-008-9564-9
ISSN
0724-8741
Abstract
Purpose. To characterize the uptake mechanism of zidovudine (AZT), a nucleoside reverse transcriptase inhibitor, in syncytiotrophoblast cells using the TR-TBT 18d-1 cell line previously established by our group. Materials and Methods. The effects of several transporter inhibitors on the initial and steady-state apical uptake of AZT by TR-TBT 18d-1 were characterized, in order to identify the transporter(s) involved. Results. Initial uptake of AZT was sodium-independent and saturable; the K(m) value was about 16 mu M. Nitrobenzylthioinosine (NBMPR), probenecid and cimetidine each had little effect on the saturable AZT uptake, indicating that well characterized transporters, such as organic anion transporters (OATs and OATPs), organic cation transporters (OCTs) and equilibrative nucleoside transporters (ENTs), are not involved. However, thymidine and 2'-deoxyuridine strongly inhibited AZT uptake. These results suggest that an unidentified nucleoside uptake transporter is responsible for the uptake of AZT. Cyclosporin A, Ko143 and probenecid had little effect on AZT accumulation by TR-TBT 18d-1 cells, suggesting that transporter-mediated efflux of AZT is not substantial. Conclusion. Our results indicate that saturable AZT uptake into TR-TBT 18d-1 is mediated by a so-far-unidentified transporter.
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