The neuronal differentiation potential of Ldb1-null mutant embryonic stem cells is dependent on extrinsic influences
- Authors
- Hwang, M.; Gorivodsky, M.; Kim, Min Jung; Westphal, H.; Geum, D.
- Issue Date
- Jun-2008
- Publisher
- AlphaMed Press Inc
- Citation
- Stem Cells, v.26, no.6, pp 1490 - 1495
- Journal Title
- Stem Cells
- Volume
- 26
- Number
- 6
- Start Page
- 1490
- End Page
- 1495
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/14244
- DOI
- 10.1634/stemcells.2007-1099
- ISSN
- 1066-5099
1549-4918
- Abstract
- LIM-domain binding protein 1 (Ldb1) is a multiadaptor protein that mediates the action of transcription factors, including LIM-homeodomain proteins. To elucidate the functional role of Ldb1 in the neuronal differentiation of embryonic stem (ES) cells, we have generated Ldb1-null mutant (Ldb1-/-) ES cells and examined neuronal differentiation potentials in vitro using two different neuronal differentiation protocols. When subjected to a five-stage protocol that recapitulates in vivo conditions of neuronal differentiation, wild-type ES cells differentiated into a wide spectrum of neuronal cell types. However, Ldb1-/- ES cells did not differentiate into neuronal cells; instead, they differentiated into sarcomeric α-actinin-positive muscle cells. In contrast, when an adherent monolayer culture procedure (which is based on the default mechanism of neural induction and eliminates environmental influences) was applied, both wild-type and Ldb1-/- ES cells differentiated into MAP2-positive mature neurons. Comparison of the results obtained when two different neuronal differentiation protocols were used suggests that Ldb1-/- ES cells have an innate potential to differentiate into neuronal cells, but this potential can be inhibited by environmental influences.
- Files in This Item
-
Go to Link
- Appears in
Collections - 이과대학 > 생명시스템학부 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.