Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

이온토포레시스를 이용한 levodopa의 경피전달: electroosmosis 및 electrorepulsion의 역할Levodopa Transport through Skin using Iontophoresis:the Role of Electroosmosis and Electrorepulsion

Other Titles
Levodopa Transport through Skin using Iontophoresis:the Role of Electroosmosis and Electrorepulsion
Authors
정신애곽혜선전인구오승열
Issue Date
Feb-2008
Publisher
한국약제학회
Keywords
Iontophoresis; Levodopa; Hydrogel; Electroosmosis; Electrorepulsion
Citation
Journal of Pharmaceutical Investigation, v.38, no.1, pp 31 - 38
Pages
8
Journal Title
Journal of Pharmaceutical Investigation
Volume
38
Number
1
Start Page
31
End Page
38
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/14532
ISSN
2093-5552
2093-6214
Abstract
The objective of this work is to study transdermal delivery of levodopa using iontophoresis and evaluate various factors which affect the transdermal transport. Levodopa is unstable in aqueous solution, and, in order to establish a stable condition for levodopa for the duration of experiment, we investigated the stability of levodopa in aqueous solutions of different pHs with/without the addition of dextrose or the application of current. Using stable aqueous solution, we have studied the effect of pH, polarity and penetration enhancer (ethanol) on transdermal flux and compared the results. We also investigated the iontophoretic flux from hydroxypropyl cellulose (HPC) hydrogel. In vitro flux study was performed at 33oC, using side-by-side diffusion cell. Full thickness hairless mouse skin and rat skin were used for this work. Current densities applied were 0.4 or 0.6 mA/cm2 and current was off after 6 hour application. Stability study showed that levodopa solution with a pH 2.5 or 4.5 maintained the initial concentration of levodopa for 24 hours with the addition of 5% dextrose. However, at pH 9.5, levodopa was unstable and 30 to 40% of levodopa degraded within 24 hours, even with the addition of 5% dextrose. Hydrogel swollen with dextrose added levodopa solution maintained about 97% of the initial concentration of levodopa for 13 days, when stored in 4oC. The application of current did not affect the stability of levodopa in hydrogel. Flux study from levodopa solution with pH 2.5 showed that cathodal delivery of levodopa was higher than passive or anodal delivery. When the pH of the donor solution was 4.5, anodal delivery of levodopa was higher than passive or cathodal delivery. These results seem to indicate that electroosmosis plays more dominant role than electrorepulsion in the flux of levodopa at pH 2.5, and the reverse situation applies for pH 4.5. The passive flux was unexpectedly high for the ionized levodopa. Similar to the results from aqueous solution, cumulative amount of levodopa transported from HPC hydrogel by cathodal delivery was significantly higher than passive or anodal delivery. The treatment of 70% ethanol cotton ball by scrubbing increased passive, anodal and cathodal flux, with the largest increase for anodal flux, These results indicate that iontophoretic delivery of zwitterion such as levodopa is much complicated than that can be expected from small ionic molecules with single charge. The results also indicate that the balance between electroosmosis and electrorepulsion plays a very important role in the transport through skin.
Files in This Item
Go to Link
Appears in
Collections
약학대학 > 약학부 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE