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Improvement of the detection efficiency of 3M™ molecular detection system for campylobacter in poultry using nitrogen-doped carbon nanodots

Authors
Ha, JimyeongSeo, YeongeunCho, EunbeeChoi, SeunghoKim, HojinYoon, Yohan
Issue Date
May-2021
Publisher
ELSEVIER
Keywords
Campylobacter; Detection; Molecular detection system; Enrichment broth; Nitrogen-doped carbon nanodots
Citation
JOURNAL OF MICROBIOLOGICAL METHODS, v.184, pp 1 - 5
Pages
5
Journal Title
JOURNAL OF MICROBIOLOGICAL METHODS
Volume
184
Start Page
1
End Page
5
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/146658
DOI
10.1016/j.mimet.2021.106211
ISSN
0167-7012
1872-8359
Abstract
This study was performed to examine whether the use of nitrogen-doped carbon nanodots (N-CNDs) can improve the detection sensitivity of the 3 M T molecular detection system (MDS) for Campylobacter. N-CNDs were added to a Campylobacter enrichment broth (CEB) at concentrations of 5 and 10 mg/mL (NCEB-5 and NCEB-10, respectively). Campylobacter coli, C. jejuni, and C. lari were inoculated into the broths. The broth cultures were then irradiated with light-emitting diode (LED) at 425 nm for 1 h and incubated at 42 degrees C for 6 h, and then grown on modified charcoal cefoperazone deoxycholate agar (mCCDA). The detection rates of the MDS and a conventional method (plating an enriched sample on mCCDA and analyzing a colony on mCCDA with PCR) for Campylobacter in chicken and duck carcasses were compared. The detection rates from the MDS were compared after enrichment in CEB and NCEB-5 at 3, 5, 6, 7, 9, 12, and 24 h. When 5 mg/mL of N-CNDs was added to the CEB followed by irradiation at 425 nm, growth of the Campylobacter was accelerated. In addition, the qualitative test was more sensitive in the MDS than in the conventional method, and the detection time was shortened in CEB enriched with N-CNDs. These results indicate that adding N-CNDs to CEB can improve the detection efficiency of MDS.
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