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Cellular functions and transcriptional regulation of a third thioredoxin from Schizosaccharomyces pombe

Authors
Kim, Su-JungJung, Eui-ManJung, Hyun-JooSong, Yun SeonPark, Eun-HeeLim, Chang-Jin
Issue Date
Jun-2007
Publisher
CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS
Keywords
fission yeast; lacZ fusion; oxidative stress; Pap1; reactive oxygen species; regulation; Schizosaccharomyces pombe; thioredoxin
Citation
CANADIAN JOURNAL OF MICROBIOLOGY, v.53, no.6, pp 775 - 783
Pages
9
Journal Title
CANADIAN JOURNAL OF MICROBIOLOGY
Volume
53
Number
6
Start Page
775
End Page
783
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/14688
DOI
10.1139/W07-040
ISSN
0008-4166
1480-3275
Abstract
The structural gene encoding a third thioredoxin (Trx) homologue, TRX3, of the fission yeast Schizosaccharomyces pombe was characterized and its regulation was studied. The determined DNA sequence encoded a putative 290 amino acid sequence of Trx with a molecular mass of 31 889 Da. The TRX3 mRNA level was increased in S. pombe cells harboring plasmid pTRX3, suggesting that the cloned TRX3 gene was functional. Yeast cultures harbouring plasmid pTRX3 exhibited shorter generation times and higher survival on solid minimal media plates incorporating mercury chloride (0.01 mmol/L) or hydrogen peroxide (1 mmol/L) compared with control cultures. Yeast cells containing extra copies of TRX3, but not TRX1 and TRX2, gave rise to lower reactive oxygen species levels than control cells. Oxidative stress owing to hydrogen peroxide and menadione enhanced the synthesis of beta-galactosidase from the TRX3-lacZ fusion gene in Pap1-positive cells but not in Pap1-negative cells. The TRX3 mRNA level was increased by oxidative stress only in Pap1-positive cells. Basal expression of the TRX3 gene also depended on Pap1. We concluded that S. pombe TRX3 is linked with yeast growth and oxidative stress response, with its expression being regulated by oxidative stress in a Pap1-dependent manner.
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