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Identification of candidate UDP-glycosyltransferases involved in protopanaxadiol-type ginsenoside biosynthesis in Panax ginseng

Authors
Kang, Kyo BinJayakodi, MurukarLee, Yun SunNguyen, Van BinhPark, Hyun-SeungKoo, Hyun JoChoi, Ik YoungKim, Dae HyunChung, You JinRyu, ByeolLee, Dong YoungSung, Sang HyunYang, Tae-Jin
Issue Date
Aug-2018
Publisher
NATURE PUBLISHING GROUP
Citation
SCIENTIFIC REPORTS, v.8, no.1, pp 1 - 10
Pages
10
Journal Title
SCIENTIFIC REPORTS
Volume
8
Number
1
Start Page
1
End Page
10
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/146918
DOI
10.1038/s41598-018-30262-7
ISSN
2045-2322
Abstract
Ginsenosides are dammarane-type or triterpenoidal saponins that contribute to the various pharmacological activities of the medicinal herb Panax ginseng. The putative biosynthetic pathway for ginsenoside biosynthesis is known in P. ginseng, as are some of the transcripts and enzyme-encoding genes. However, few genes related to the UDP-glycosyltransferases (UGTs), enzymes that mediate glycosylation processes in final saponin biosynthesis, have been identified. Here, we generated three replicated Illumina RNA-Seq datasets from the adventitious roots of P. ginseng cultivar Cheongsun (CS) after 0, 12, 24, and 48 h of treatment with methyl jasmonate (MeJA). Using the same CS cultivar, metabolomic data were also generated at 0 h and every 12–24 h thereafter until 120 h of MeJA treatment. Differential gene expression, phylogenetic analysis, and metabolic profiling were used to identify candidate UGTs. Eleven candidate UGTs likely to be involved in ginsenoside glycosylation were identified. Eight of these were considered novel UGTs, newly identified in this study, and three were matched to previously characterized UGTs in P. ginseng. Phylogenetic analysis further asserted their association with ginsenoside biosynthesis. Additionally, metabolomic analysis revealed that the newly identified UGTs might be involved in the elongation of glycosyl chains of ginsenosides, especially of protopanaxadiol (PPD)-type ginsenosides.
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