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The gene expression profiling in murine cortical cells undergoing programmed cell death (PCD) induced by serum deprivation

Authors
Yang, Moon HeeYoo, Kyung HyunYook, Yeon JooPark, Eun YoungJeon, Jeong OkChoi, Seo HeePark, So YoungWoo, Yu MiLee, Min JooPark, Jong Hoon
Issue Date
Mar-2007
Publisher
KOREAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY
Keywords
apoptosis; cortical cells; cDNA microarray; screening; UNC-51-like kinase (ULK) 2
Citation
JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, v.40, no.2, pp 277 - 285
Pages
9
Journal Title
JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY
Volume
40
Number
2
Start Page
277
End Page
285
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/14720
DOI
10.5483/BMBRep.2007.40.2.277
ISSN
1225-8687
1976-670X
Abstract
PCD (programmed cell death) is important mechanism for development, homeostasis and disease. To analyze the gene expression pattern in brain cells undergoing PCD in response to serum deprivation, we analyzed the cDNA microarray consisting of 2,300 genes and 7 housekeeping genes of cortical cells derived from mouse embryonic brain. Cortical cells were induced apoptosis by serum deprivation.for 8 hours. We identified 69 up-regulated genes and 21 down-regulated genes in apoptotic cells. Based on the cDNA microarray data four genes were selected and analyzed by RT-PCR and northern blotting. To characterize the role of UNC-51-like kinase (ULK2) gene in PCD, we investigated cell death effect by ULK2. And we examined expression of several genes that related with PCD. Especially GAPDH was increased by ULK2. Theses findings indicated that ULK2 is involved in apoptosis through p53 pathway.
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