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Photodegradable Hydrogels for Capture, Detection, and Release of Live Cells

Authors
Shin, DS (Shin, Dong-Sik)You, J (You, Jungmok)Rahimian, A (Rahimian, Ali)Vu, T (Tam Vu)Siltanen, C (Siltanen, ChristiEhsanipour, A (Ehsanipour, ArsStybayeva, G (Stybayeva, GulnaSutcliffe, J (Sutcliffe, JulieRevzin, A (Revzin, Alexander)
Issue Date
Jul-2014
Publisher
WILEY-V C H VERLAG GMBH
Citation
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, v.53, no.31, pp 8221 - 8224
Pages
4
Journal Title
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume
53
Number
31
Start Page
8221
End Page
8224
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/147290
DOI
10.1002/anie.201404323
ISSN
1433-7851
1521-3773
Abstract
Cells may be captured and released using a photodegradable hydrogel (photogel) functionalized with antibodies. Photogel substrates were used to first isolate human CD4 or CD8 T-cells from a heterogeneous cell suspension and then to release desired cells or groups of cells by UV-induced photodegradation. Flow cytometry analysis of the retrieved cells revealed approximately 95% purity of CD4 and CD8 T-cells, suggesting that this substrate had excellent specificity. To demonstrate the possibility of sorting cells according to their function, photogel substrates that were functionalized with anti-CD4 and anti-TNF-alpha antibodies were prepared. Single cells captured and stimulated on such substrates were identified by the fluorescence "halo" after immunofluorescent staining and could be retrieved by site-specific exposure to UV light through a microscope objective. Overall, it was demonstrated that functional photodegradable hydrogels enable the capture, analysis, and sorting of live cells.
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공과대학 (화공생명공학부)
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