Photodegradable Hydrogels for Capture, Detection, and Release of Live Cells
- Authors
- Shin, DS (Shin, Dong-Sik); You, J (You, Jungmok); Rahimian, A (Rahimian, Ali); Vu, T (Tam Vu); Siltanen, C (Siltanen, Christi; Ehsanipour, A (Ehsanipour, Ars; Stybayeva, G (Stybayeva, Gulna; Sutcliffe, J (Sutcliffe, Julie; Revzin, A (Revzin, Alexander)
- Issue Date
- Jul-2014
- Publisher
- WILEY-V C H VERLAG GMBH
- Citation
- ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, v.53, no.31, pp 8221 - 8224
- Pages
- 4
- Journal Title
- ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
- Volume
- 53
- Number
- 31
- Start Page
- 8221
- End Page
- 8224
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/147290
- DOI
- 10.1002/anie.201404323
- ISSN
- 1433-7851
1521-3773
- Abstract
- Cells may be captured and released using a photodegradable hydrogel (photogel) functionalized with antibodies. Photogel substrates were used to first isolate human CD4 or CD8 T-cells from a heterogeneous cell suspension and then to release desired cells or groups of cells by UV-induced photodegradation. Flow cytometry analysis of the retrieved cells revealed approximately 95% purity of CD4 and CD8 T-cells, suggesting that this substrate had excellent specificity. To demonstrate the possibility of sorting cells according to their function, photogel substrates that were functionalized with anti-CD4 and anti-TNF-alpha antibodies were prepared. Single cells captured and stimulated on such substrates were identified by the fluorescence "halo" after immunofluorescent staining and could be retrieved by site-specific exposure to UV light through a microscope objective. Overall, it was demonstrated that functional photodegradable hydrogels enable the capture, analysis, and sorting of live cells.
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