Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Characterizing the Effects of Heparin Gel Stiffness on Function of Primary Hepatocytes

Authors
You, J (You, Jungmok)Park, SA (Park, Su-A)Shin, DS (Shin, Dong-Sik)Patel, D (Patel, Dipali)Raghunathan, VK (Raghunathan,Kim, M (Kim, Mihye)Murphy, CJ (Murphy, ChristopheTae, G (Tae, Giyoong)Revzin, A (Revzin, Alexander)
Issue Date
Dec-2013
Publisher
MARY ANN LIEBERT
Citation
TISSUE ENGINEERING PART A, v.19, no.23-24, pp.2655 - 2663
Journal Title
TISSUE ENGINEERING PART A
Volume
19
Number
23-24
Start Page
2655
End Page
2663
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/147392
DOI
10.1089/ten.tea.2012.0681
ISSN
1937-3341
Abstract
In the liver, hepatocytes are exposed to a large array of stimuli that shape hepatic phenotype. This in vivo microenvironment is lost when hepatocytes are cultured in standard cell cultureware, making it challenging to maintain hepatocyte function in vitro. Our article focused on one of the least studied inducers of the hepatic phenotypethe mechanical properties of the underlying substrate. Gel layers comprised of thiolated heparin (Hep-SH) and diacrylated poly(ethylene glycol) (PEG-DA) were formed on glass substrates via a radical mediated thiol-ene coupling reaction. The substrate stiffness varied from 10 to 110kPa by changing the concentration of the precursor solution. ELISA analysis revealed that after 5 days, hepatocytes cultured on a softer heparin gel were synthesizing five times higher levels of albumin compared to those on a stiffer heparin gel. Immunofluorescent staining for hepatic markers, albumin and E-cadherin, confirmed that softer gels promoted better maintenance of the hepatic phenotype. Our findings point to the importance of substrate mechanical properties on hepatocyte function.
Files in This Item
There are no files associated with this item.
Appears in
Collections
공과대학 > 화공생명공학부 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Shin, Dong Sik photo

Shin, Dong Sik
공과대학 (화공생명공학부)
Read more

Altmetrics

Total Views & Downloads

BROWSE