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Modeling and optimization of lipase-catalyzed synthesis of phytosteryl esters of oleic acid by response surface methodology

Authors
Kim, Byung HeeAkoh, Casimir C.
Issue Date
Jan-2007
Publisher
Elsevier BV
Keywords
Candida rugosa lipase; Esterification; Phytosterols; Phytosteryl esters of fatty acids; Response surface methodology
Citation
Food Chemistry , v.102, no.1, pp 336 - 342
Pages
7
Journal Title
Food Chemistry
Volume
102
Number
1
Start Page
336
End Page
342
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/148477
DOI
10.1016/j.foodchem.2006.05.025
ISSN
0308-8146
1873-7072
Abstract
Enzymatic esterification of phytosterols with oleic acid to produce phytosteryl esters was performed in hexane. Response surface methodology was used to model the reaction. Candida rugosa lipase was the biocatalyst for the reaction. The reaction factors investigated were temperature (Te = 35-55 degrees C, reaction time (t = 4-24 h), substrate molar ratio (Sr = 1-3, oleic acid: phyto sterols). and enzyme amount (En = 2-10%). Well-fitting quadratic polynomial regression model for degree of esterification (DE) was established after regression analysis with backward elimination and verified by a chi(2) test. All factors investigated positively affected DE, with t having the greatest effect followed by En, Sr, and Te. The quadratic terms of t, Sr, and En showed negative effects on DE, whereas, that of Te had no effect on DE. Optimal reaction conditions were: Te, 51.3 degrees C; t, 17.0 h; Sr, 2. 1; En, 7.2% and DE was 97.0 mol% under these conditions. (c) 2006 Elsevier Ltd. All rights reserved.
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생활과학대학 (식품영양학과)
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