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Fluorescent protein expression driven by her4 regulatory elements reveals the spatiotemporal pattern of Notch signaling in the nervous system of zebrafish embryos

Authors
Yeo, Sang-YeobKim, MinJungKim, Hyung-SeokHuh, Tae-LinChitnis, Ajay B.
Issue Date
Jan-2007
Publisher
Academic Press
Keywords
CBF1; CSL; her4; mind bomb; Neurogenesis; Neurogenin1; Notch reporter; Su(H); Transgenic; zath3
Citation
Developmental Biology, v.301, no.2, pp 555 - 567
Pages
13
Journal Title
Developmental Biology
Volume
301
Number
2
Start Page
555
End Page
567
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/148478
DOI
10.1016/j.ydbio.2006.10.020
ISSN
0012-1606
1095-564X
Abstract
Notch activation inhibits neuronal differentiation during development of the nervous system; however, the dynamic role of Notch signaling in individual cell lineages remains poorly understood. We have characterized 3.4 kb 5'-regulatory sequence of a Notch target gene, her4, and used it to drive fluorescent gene expression in transgenic lines where the spatiotemporal pattern of Notch activation can be examined in vivo. The 3.4 kb her4 promoter contains five predicted Su(H) binding sites of which two proximal sites were confirmed to be required for Notch-mediated transcriptional activation. Without Notch, Su(H) effectively represses transcription regulated by the promoter. Analyses of transgenic zebrafish showed that while the expression of proneural genes and Notch activation were both critical for endogenous her4 expression, reporter gene expression was primarily regulated by Notch activity. This study also showed that her4 may be differently regulated in sensory cranial ganglia, where Notch activity is not essential for her4 expression and where Su(H) may repress her4 expression. The establishment of a reporter line with Notch-Su(H)-dependent fluorescent gene expression provides a tool to explore the complex role of Notch signaling in the development of vertebrate nervous system.(신경계에서 Notch의 activation이 신경분화를
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