Production of vanillin by metabolically engineered Escherichia coli
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoon, SH | - |
dc.contributor.author | Li, C | - |
dc.contributor.author | Kim, JE | - |
dc.contributor.author | Lee, SH | - |
dc.contributor.author | Yoon, JY | - |
dc.contributor.author | Choi, MS | - |
dc.contributor.author | Seo, WT | - |
dc.contributor.author | Yang, JK | - |
dc.contributor.author | Kim, JY | - |
dc.contributor.author | Kim, SW | - |
dc.date.accessioned | 2022-04-19T11:44:01Z | - |
dc.date.available | 2022-04-19T11:44:01Z | - |
dc.date.issued | 2005-11 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.issn | 1573-6776 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/148829 | - |
dc.description.abstract | E. coli was metabolically engineered to produce vanillin by expression of the fcs and ech genes from Amycolatopsis sp. encoding feruloyl-CoA synthetase and enoyl-CoA hydratase/aldolase, respectively. Vanillin production was optimized by leaky expression of the genes, under the IPTG-inducible trc promoter, in complex 2YT medium. Supplementation with glucose, fructose, galactose, arabinose or glycerol severely decreased vanillin production. The highest vanillin production of 1.1 g l(-1) was obtained with cultivation for 48 h in 2YT medium with 0.2% (w/v) ferulate, without IPTG and no supplementation of carbon sources. | - |
dc.format.extent | 4 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER | - |
dc.title | Production of vanillin by metabolically engineered Escherichia coli | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1007/s10529-005-3561-4 | - |
dc.identifier.scopusid | 2-s2.0-28344434521 | - |
dc.identifier.wosid | 000233634500016 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY LETTERS, v.27, no.22, pp 1829 - 1832 | - |
dc.citation.title | BIOTECHNOLOGY LETTERS | - |
dc.citation.volume | 27 | - |
dc.citation.number | 22 | - |
dc.citation.startPage | 1829 | - |
dc.citation.endPage | 1832 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Ferulic acid | - |
dc.subject.keywordAuthor | Metabolic engineering | - |
dc.subject.keywordAuthor | Recombinant E. coli | - |
dc.subject.keywordAuthor | Vanillin | - |
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