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Heat shock factor regulates VDUP1 gene expression

Authors
Kim, KYKim, JKShin, SMPaik, SGYang, YChoi, I
Issue Date
Mar-2004
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Keywords
HST; Promoter; Stress; VDUP1
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.315, no.2, pp 369 - 375
Pages
7
Journal Title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume
315
Number
2
Start Page
369
End Page
375
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/148970
DOI
10.1016/j.bbrc.2004.01.047
ISSN
0006-291X
1090-2104
Abstract
The vitamin D3 up-regulated protein 1 (VDUP1) is identified as interacting protein with thioredoxin (TRX) and functions as a natural antagonist of TRX. Its expression is regulated by various stresses including ROS, UV, and heat shock. In the present study, we observed an inducible expression of VDUP1 in Bosc cells by high density and serum deprivation cultures. To determine transcription factors associated with the induction of VDUP1 by stresses, the promoter region of VDUP1 was cloned. Through reporter assays with plasmids having various deletion of its promoter region and analysis of putative cis-elements, heat shock factor element (HSE) was identified. The deletion of HSE abolished transcriptional activity of VDUP1 promoter by stresses and the binding of heat shock factor (HSF) to HSE was confirmed by gel-shift and supershift assays using nuclear extracts prepared from stressed Bosc cells. Also, the enforced expression of HSF or heat shock increased the transcription of endogenous VDUP1 These imply that HSF is an important transcription factor involved in up-regulation of VDUP1 expression by stresses such as high density and serum deprivation cultures. (C) 2004 Elsevier Inc. All rights reserved.
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