Protein patterning by maskless photolithography on hydrophilic polymer-grafted surface
- Authors
- Shin, DS; Lee, KN; Janga, KH; Kim, JK; Chung, WJ; Kim, YK; Lee, YS
- Issue Date
- Dec-2003
- Publisher
- ELSEVIER ADVANCED TECHNOLOGY
- Keywords
- Maskless photolithography; Micromirror array; Nonspecific binding; Polymer-grafted surface; Protein chip
- Citation
- BIOSENSORS & BIOELECTRONICS, v.19, no.5, pp 485 - 494
- Pages
- 10
- Journal Title
- BIOSENSORS & BIOELECTRONICS
- Volume
- 19
- Number
- 5
- Start Page
- 485
- End Page
- 494
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/149046
- DOI
- 10.1016/S0956-5663(03)00228-8
- ISSN
- 0956-5663
1873-4235
- Abstract
- With the help of a microfabrication process and surface modification technology, a method of fabricating protein patterned chips was developed which can be utilized as a powerful tool for performing bioassays in a high-throughput manner. A digital micromirror array (MMA) system was used as a virtual photomask, so that a maskless photolithography process was able to be used to build patterned biomolecules on a chip by selective illumination onto the chip surface. We utilized the nitroveratryloxycarbonyl (NVOC) group as a photolabile protecting group for protein patterning. The NVOC-protected surface was selectively irradiated by a UV illuminator using an MMA. After removing the NVOC group, biotin was coupled to the NVOC-cleaved site, onto which a buffered streptavidin solution was eluted. At this point, we could obtain a streptavidin-patterned surface and observe the effect of the polymer-grafted surface in reducing nonspecific binding. (C) 2003 Elsevier B.V. All rights reserved.
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