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Dexamethasone reverses TGF-beta-mediated inhibition of primary rat preadipocyte differentiation

Authors
Shin, SMKim, KYKim, JKYoon, SRChoi, IYang, Y
Issue Date
May-2003
Publisher
ELSEVIER SCIENCE BV
Keywords
CCAAT/enhancer binding protein-α; Dexamethasone; Peroxisome proliferator-activated receptor γ; Rat preadipocyte; Transforming growth factor-β
Citation
FEBS LETTERS, v.543, no.1-3, pp 25 - 30
Pages
6
Journal Title
FEBS LETTERS
Volume
543
Number
1-3
Start Page
25
End Page
30
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/149159
DOI
10.1016/S0014-5793(03)00371-5
ISSN
0014-5793
1873-3468
Abstract
Dexamethasone and transforming growth factor-beta (TGF-beta) show contrary effects on differentiation of adipocytes. Dexamethasone stimulates adipocyte differentiation whereas TGF-beta inhibits it. In the present study, we investigated whether dexamethasone could reverse the TGF-beta-mediated inhibition of preadipocyte differentiation. Primary rat preadipocytes, obtained from Sprague-Dawley rats, were pretreated with dexamethasone in the presence or absence of TGF-beta, prior to the induction of differentiation. Co-treatment of dexamethasone and TGF-beta before inducing differentiation reversed the TGF-beta-mediated inhibition of preadipocyte differentiation. In order to elucidate the mechanism by which dexamethasone reversed the effect of TGF-beta on the inhibition of preadipocyte differentiation, the expression of CCAAT/enhancer binding protein-alpha (C/EBPalpha) and peroxisome proliferator-activated receptorgamma(PPARgamma) was examined. Dexamethasone increased C/EBPalpha and PPARgamma expression in the absence of TGF-beta and also recovered the TGF-beta-mediated suppression of C/EBPalpha expression in preadipocytes. Its effect was sustained in differentiated adipocytes as well. However, those effects were not observed in 3T3-L1 preadipocytes or differentiated adipocytes. These results indicate that dexametha
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