Enhanced antigen-presenting activity and tumour necrosis factor-alpha-independent activation of dendritic cells following treatment with Mycobacterium bovis bacillus Calmette-Guerin
- Authors
- Kim, KD; Lee, HG; Kim, JK; Park, SN; Choe, IS; Choe, YK; Kim, SJ; Lee, E; Lim, JS
- Issue Date
- Aug-1999
- Publisher
- BLACKWELL PUBLISHING
- Citation
- IMMUNOLOGY, v.97, no.4, pp 626 - 633
- Pages
- 8
- Journal Title
- IMMUNOLOGY
- Volume
- 97
- Number
- 4
- Start Page
- 626
- End Page
- 633
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/150176
- DOI
- 10.1046/j.1365-2567.1999.00818.x
- ISSN
- 0019-2805
1365-2567
- Abstract
- Dendritic cells (DCs) are mast potent among the antigen-presenting cells and are believed to be crucial for the initiation of a primary T-cell response to foreign antigens. Mycobacterial infection within macrophages is controlled by cell-mediated immunity. To elucidate the stimulation of immune response by Mycobacterium bovis bacillus Calmette-Guerin (BCG), we purified DCs from precursor cells in human peripheral blood mononuclear cells (PBMC) by culturing them with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) and characterized their surface antigen expression. The interaction of cultured DCs with BCC resulted in increased surface expression of several DC-related marker antigens. BCG also induced reduction of endocytosis, enhancement of CD83 expression as well as B7 costimulatory molecules and IL-12 production, suggesting that BCG treatment directly induces DCs to mature. BCG-treated DCs were much more potent antigen-presenting cells in allogeneic immune response than untreated DCs. Moreover, while the neutralization of tumour necrosis factor-alpha (TNF-alpha) significantly blocked the DC maturation induced by lipopolysaccharide (LPS), it could not inhibit the induction of DC maturation by the BCG treatment, indicating that TNF-alpha production plays a minor role in the BCC-
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