Polyethylene glycol-grafted poly-l-lysine as polymeric gene carrier
- Authors
- Young Hun Choi; Feng Liu; Jin-Seok Kim; Young Kweon Choi; Jong Sang Park; Sung Wan Kim
- Issue Date
- Jun-1998
- Publisher
- Elsevier BV
- Citation
- Journal of Controlled Release, v.54, no.1, pp 39 - 48
- Pages
- 10
- Journal Title
- Journal of Controlled Release
- Volume
- 54
- Number
- 1
- Start Page
- 39
- End Page
- 48
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/150360
- DOI
- 10.1016/S0168-3659(97)00174-0
- ISSN
- 0168-3659
1873-4995
- Abstract
- A new series of gene carriers, polyethylene glycol (PEG)-grafted poly-l-lysine (PLL, mol. wt.=25 000) with three different PEG-grafted ratios (5, 10 and 25 mole%, which means 5, 10 and 25% of ϵ-amino group of PLL was modified by PEG), was synthesized. These new gene carriers, named comb-shaped PEG-g-PLL copolymer, showed a 5- to 30-fold increase in transfection efficiency compared to PLL alone on a human carcinoma cell line. It is likely that Hep G2 cells were transfected by plasmid DNA/PEG-g-PLL complexes through an endocytosis mechanism due to the fact that chloroquine increased transfection efficiency. Although Lipofectin™, a cationic lipid formulation, showed slightly higher transfection efficiency than PEG-g-PLL in Hep G2 cells, our designed PEG-g-PLL demonstrated lower cytotoxicity, early gene expression and maintenance of gene expression for up to 96 h.
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