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Complete sequence of the upp gene encoding uracil phosphoribosyltransferase from Mycobacterium bovis BCG

Authors
Kim, JKKim, JHLee, SHKang, YLim, JSLee, HGKim, SJBai, GHPark, YKCho, SHKang, SWKim, YSChoe, ISChung, TWPark, SNKim, SHAhn, JSChoe, YK
Issue Date
May-1997
Publisher
Academic Press
Citation
Biochemistry and Molecular Biology International, v.41, no.6, pp 1117 - 1124
Pages
8
Journal Title
Biochemistry and Molecular Biology International
Volume
41
Number
6
Start Page
1117
End Page
1124
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/150577
DOI
10.1080/15216549700202201
ISSN
1039-9712
Abstract
The nucleotide sequence of a 799 bp fragment of Mycobacterium bovis BCG containing the putative upp gene that encodes a uracil phosphoribosyltransferase (UPRTase [EC 2.4.2.9]) has been determined. The upp gene of BCG has an open reading frame (ORF) of 621 bp (207 amino acids) starting with GTG (position 112) and ending with TGA (position 733), and its molecular mass was calculated to be 21,864 Da. Comparative analyses of the deduced amino acid sequence of BCG UPRTase with the UPRTase of six bacterial genera revealed that 24% (50/211) of the residues are perfectly conserved and 32% (67/211) of the residues are well conserved.
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