Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

마우스(H-2Kb)에서 간염 바이러스 핵 항원에 의한 CTL유도Induction of H-2Kb-restricted cytotoxic T lymphocyte on hepatitis B virus core antigen

Other Titles
Induction of H-2Kb-restricted cytotoxic T lymphocyte on hepatitis B virus core antigen
Authors
김재화이희구이기영임종석김용호박순희최용경정태화최인성김길현
Issue Date
Jun-1996
Publisher
대한면역학회
Citation
대한면역학회지(Korean Journal of Immunology), v.18, no.2, pp 237 - 245
Pages
9
Journal Title
대한면역학회지(Korean Journal of Immunology)
Volume
18
Number
2
Start Page
237
End Page
245
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/150739
ISSN
1015-6453
Abstract
Recently reported viral peptides from vims-infected cells that are bound to class I MHC molecules showed 8-10mer sequences and typical dominant anchor residues. Using the mutant tumor line RMA-S, which expresses its surface H-2$$K^b$$ molecules devoid of peptides, we investigated the specific CTL epitopes on the hepatitis B viral surface and core proteins that were isolated from hepatitis B virus-infected patients. This was done by examining the ability of randomly trypsinized protein to bind to H-2$$K^b$$ or $$D^b$$ molecule on RMA-S cells. The trypsin-treated proteins enhanced the expression of H-$$2^b$$ molecules on RMA-S cells. We have analyzed the digested proteins by high performance liquid chromatography, and binding assays with respective peptide fractions showed the presence of H-$$2^b$$ binding epitopes on hepatits B viral protein. These findings suggest that it is possible to determine the epitope sequences directly by measuring their binding to class I molecules followed by analyzing their amino acid sequences.
Files in This Item
Go to Link
Appears in
Collections
이과대학 > 생명시스템학부 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Lim, Jong Seok photo

Lim, Jong Seok
이과대학 (생명시스템학부)
Read more

Altmetrics

Total Views & Downloads

BROWSE