Effects of heterologous expression of thioredoxin reductase on the level of reactive oxygen species in COS-7 cells
- Authors
- Kang, Hyun-Jung; Hong, Sung-Min; Kim, Byung-Chul; Park, Eun-Hee; Ahn, Kisup; Lim, Chang-Jin
- Issue Date
- Aug-2006
- Publisher
- KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
- Keywords
- COS-7; hydrogen peroxide; reactive oxygen species; thioredoxin reductase
- Citation
- MOLECULES AND CELLS, v.22, no.1, pp 113 - 118
- Pages
- 6
- Journal Title
- MOLECULES AND CELLS
- Volume
- 22
- Number
- 1
- Start Page
- 113
- End Page
- 118
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/15078
- ISSN
- 1016-8478
0219-1032
- Abstract
- Thioredoxin reductase (TrxR), a component of the redox control system involving thioredoxin (Trx), is implicated in defense against oxidative stress, control of cell growth and proliferation, and regulation of apoptosis. In the present study a stable transfectant was made by introducing the vector pcDNA3.0 harboring the fission yeast TrxR gene into COS-7 African green monkey kidney fibroblast cells. The exogenous TrxR gene led to an increase in TrxR activity of up to 3.2-fold but did not affect glutathione (GSH) content, or glutaredoxin and caspase-3 activities. Levels of reactive oxygen species (ROS), but not those of nitric oxide (NO), were reduced. Conversely, 1-chloro-2,4-dinitrobezene (CDNB), an irreversible inhibitor of mammalian TrxR, enhanced ROS levels in the COS-7 cells. After treatment with hydrogen peroxide, the level of intracellular ROS was lower in the transfectants than in the vector control cells. These results confirm that TrxR is a crucial determinant of the level of cellular ROS during oxidative stress as well as in the normal state.
- Files in This Item
-
Go to Link
- Appears in
Collections - 약학대학 > 약학부 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.