IL-18 enhances thrombospondin-1 production in human gastric cancer via JNK pathway
- Authors
- Kim, Jihye; Kim, Cherlhyun; Kim, Tae Sung; Bang, Sa Ik; Yang, Young; Park, Hyunjeong; Cho, Daeho
- Issue Date
- Jun-2006
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- IL-18; TSP-1; gastric cancer; JNK pathway
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.344, no.4, pp 1284 - 1289
- Pages
- 6
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 344
- Number
- 4
- Start Page
- 1284
- End Page
- 1289
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/15114
- DOI
- 10.1016/j.bbrc.2006.04.016
- ISSN
- 0006-291X
1090-2104
- Abstract
- IL-18 is a pleiotropic cytokine that is produced by many cancer cells. A recent report suggested that IL-18 plays a key role in regulating the immune escape of melanoma and gastric cancer cells. Thrombospondin (TSP-1) is known to inhibit angiogenesis in several cancers but some studies have reported that it stimulates angiogenesis in some cancers such as gastric cancer. IL-18 and TSP-1 are related to tumor proliferation and metastasis. This study investigated the relationship between IL-18 and TSP-1 in gastric cancer. RT-PCR and ELISA showed that after the cells had been treated with IL-18, the level of TSP-1 mRNA expression and TSP-1 protein production by IL-18 increased in both a dose- and time-dependent manner. The cells were next treated with specific inhibitors in order to determine the signal pathway involved in IL-18-enhanced TSP-1 production. IL-18-enhanced TSP-1 expression was blocked by SP600125, a c-Jun N-terminal kinase (JNK) specific inhibitor. In addition, Western blot showed that IL-18 enhanced the expression of phosphorylated JNK. Overall, these results suggest that IL-18 plays a key role in TSP-1 expression involving JNK. (c) 2006 Elsevier Inc. All rights reserved.
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