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Maintenance of a plant line containing an antisense gene and silencing of the target gene following sexual crosses

Authors
천충일Delauney, Ashton J.Verma, Desh Pal S.
Issue Date
Jan-1990
Publisher
Elsevier BV
Citation
Plant Science, v.66, no.2, pp 231 - 236
Pages
6
Journal Title
Plant Science
Volume
66
Number
2
Start Page
231
End Page
236
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/151207
DOI
10.1016/0168-9452(90)90208-6
ISSN
0168-9452
Abstract
Doubly transformed tobacco plants containing both sense and antisense chloramphenicol aceltyltransferase (CAT) genes and showing > 90% inhibition of CAT gene expression were previously produced. One such plant was back-crossed with untransformed tobacco, and progeny were selected in which the CAT and anti-CAT genes were segregated. Progency containing the CAT gene but lacking the antisense construct showed fully restored CAT activity whereas those containing only the antisense genes expressed no CAT activity. The latter were stably maintained through two generations. Recombination of the sense and antisense genes by sexual crossing resulted in efficient antisense RNA-mediated inactivation of the CAT gene. © 1990.
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