Maintenance of a plant line containing an antisense gene and silencing of the target gene following sexual crosses
- Authors
- 천충일; Delauney, Ashton J.; Verma, Desh Pal S.
- Issue Date
- Jan-1990
- Publisher
- Elsevier BV
- Citation
- Plant Science, v.66, no.2, pp 231 - 236
- Pages
- 6
- Journal Title
- Plant Science
- Volume
- 66
- Number
- 2
- Start Page
- 231
- End Page
- 236
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/151207
- DOI
- 10.1016/0168-9452(90)90208-6
- ISSN
- 0168-9452
- Abstract
- Doubly transformed tobacco plants containing both sense and antisense chloramphenicol aceltyltransferase (CAT) genes and showing > 90% inhibition of CAT gene expression were previously produced. One such plant was back-crossed with untransformed tobacco, and progeny were selected in which the CAT and anti-CAT genes were segregated. Progency containing the CAT gene but lacking the antisense construct showed fully restored CAT activity whereas those containing only the antisense genes expressed no CAT activity. The latter were stably maintained through two generations. Recombination of the sense and antisense genes by sexual crossing resulted in efficient antisense RNA-mediated inactivation of the CAT gene. © 1990.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 이과대학 > 생명시스템학부 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.