Quantitative analysis by diffuse reflectance infrared Fourier transform and linear stepwise multiple regression analysis I -Simultaneous quantitation of ethenzamide, isopropylantipyrine, caffeine, and allylisopropylacetylurea in tablet by DRIFT and linear stepwise multiple regression analysis-

Abstract

Quantitation of ethenzamide, isopropylantipyrine and caffeine takes about 41 hrs by conventional GC method. Quantitation of allylisopropylacetylurea takes about 40 hrs by conventional UV method. But quantitation of them takes about 6 hrs by DRIFT developing method. Each standard and sample sieved, powdered and acquired DRIFT spectrum. Out of them peak of each component was selected and ratio of each peak to standard peak was acquired, and then linear stepwise multiple regression was performed with these data and concentration. Reflectance value, Kubelka-Munk equation and Inverse-Kubelka-Munk equation were modified by us. Inverse-Kubelka-Munk equation completed the deficit of Kubelka-Munk equation. Correlation coefficients acquired by conventional GC and UV against DRIFT were more than 0.95.