Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Development of Listeria monocytogenes detection technique in mushroom based on real-time quantitative PCR through improvement of enrichment medium

Authors
Kim, WooriYoon, YohanSeo, YeongeunLee, YewonLee, HeeyoungKim, SejeongHa, JimyeongChoi, YukyungOh, HyeminKim, YujinKang, JoohyunPark, EunyoungYoo, YoonjeongSung, MiseonLee, Soomin
Issue Date
Nov-2021
Publisher
JAPANESE SOC FOOD SCI & TECHNOLOGY
Keywords
real-time PCR; detection system; enrichment medium; mushroom; Listeria monocytogenes
Citation
FOOD SCIENCE AND TECHNOLOGY RESEARCH, v.27, no.6, pp.837 - 846
Journal Title
FOOD SCIENCE AND TECHNOLOGY RESEARCH
Volume
27
Number
6
Start Page
837
End Page
846
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/151422
DOI
10.3136/fstr.27.837
ISSN
1344-6606
Abstract
The objective of this study was to develop precise and rapid detection methods for Listeria monocytogenes in mushrooms when using real-time PCR. L. monocytogenes were enriched through the optimization of the enrichment broth. To determine the optimal supplement for bacterial growth, the various components were investigated. As a result, LEB with 2 xferric citrate (LEB+2FC) was shown the most effective on promoting the L. monocytogenes growth in mushrooms. L. monocytogenes was inoculated in King Oyster mushroom and Golden needle mushroom, and the samples were enriched. Consequently, L. monocytogenes in King Oyster mushroom was detected by 100 % (8/8) using real-time PCR when enriched in LEB+2FC for 9 h. In Golden needle mushroom, the pathogen was detected (100 %; 8/8) when enriched in LEB+2FC for 3 h. These results indicate that L. monocytogenes can be detected quickly to prevent Listeria-related foodborne illness from the consumption of mushrooms with enrichment using LEB+2FC and real-time PCR analysis.
Files in This Item
There are no files associated with this item.
Appears in
Collections
생활과학대학 > 식품영양학과 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Yoon, Yo Han photo

Yoon, Yo Han
생활과학대학 (식품영양학과)
Read more

Altmetrics

Total Views & Downloads

BROWSE