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Bifidobacterium bifidum BGN4 Paraprobiotic Supplementation Alleviates Experimental Colitis by Maintaining Gut Barrier and Suppressing Nuclear Factor Kappa B Activation Signaling Molecules

Authors
Lee, So-YoungLee, Byung-HooPark, Jong-HyunPark, Myeong-SooJi, Geun-EogSung, Mi-Kyung
Issue Date
Feb-2022
Publisher
한국식품영양과학회
Keywords
Bifidobacterium bifidum; inflammatory bowel diseases; intestinal barrier; paraprobiotics
Citation
Journal of Medicinal Food, v.25, no.2, pp 146 - 157
Pages
12
Journal Title
Journal of Medicinal Food
Volume
25
Number
2
Start Page
146
End Page
157
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/151429
DOI
10.1089/jmf.2021.K.0150
ISSN
1096-620X
1557-7600
Abstract
Inflammatory bowel diseases, including Crohn's disease and ulcerative colitis, are characterized by chronic gastrointestinal inflammation with continuous relapse-remission cycles. This study aimed to evaluate the protective effect of Bifidobacterium bifidum BGN4 as a probiotic or paraprobiotic against dextran sulfate sodium (DSS)-induced colitis in mice. Ten-week-old female BALB/c mice were randomly divided into five groups. The control (CON) and DSS groups received oral gavage of PBS, whereas the live B. bifidum (LIVE), heat-killed B. bifidum BGN4 (HEAT), and lysozyme-treated B. bifidum BGN4 (LYSOZYME) groups received live B. bifidum BGN4, heat-killed B. bifidum BGN4, and lysozyme-treated B. bifidum BGN4, respectively, for 10 days, followed by DSS supply to induce colitis. The paraprobiotic (HEAT and LYSOZYME) groups had less body weight loss and colon length shortening than the DSS or LIVE groups. The LYSOZYME group exhibited better preserved intestinal barrier integrity than the LIVE group by upregulating gap junction protein expression possibly through activating NOD-like receptor family pyrin domain containing 6/caspase-1/interleukin (IL)-18 signaling. The LYSOZYME group showed downregulated proinflammatory molecules, including p-inhibitor of kappa B proteins alpha (IκBα), cycloxygenase 2 (COX2), IL-1β, and T-bet, whereas the expression of the regulatory T cell transcription factor, forkhead box P3 expression, was increased. The paraprobiotic groups showed distinct separation of microbiota distribution and improved inflammation-associated dysbiosis. These results suggest that B. bifidum BGN4 paraprobiotics, especially lysozyme-treated BGN4, have a preventive effect against DSS-induced colitis, impacting intestinal barrier integrity, inflammation, and dysbiosis. Copyright © 2022 Mary Ann Liebert, Inc.
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생활과학대학 (식품영양학과)
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