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Transcriptional induction of cyclooxygenase-2 in osteoclast precursors is involved in RANKL-induced osteoclastogenesis

Authors
Han, SYLee, NKKim, KHJang, IWYim, MKim, JHLee, WJLee, SY
Issue Date
Aug-2005
Publisher
AMER SOC HEMATOLOGY
Citation
BLOOD, v.106, no.4, pp 1240 - 1245
Pages
6
Journal Title
BLOOD
Volume
106
Number
4
Start Page
1240
End Page
1245
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/15484
DOI
10.1182/blood-2004-12-4975
ISSN
0006-4971
1528-0020
Abstract
Regulation of osteoclast differentiation is key to understanding the pathogenesis and to developing treatments for bone diseases such as osteoporosis. To gain insight into the mechanism of the receptor activator of nuclear factor (NF)-kappa B ligand (RANKL)-specific induction of the osteoclast differentiation program, we took a suppression-subtractive hybridization screening approach to identify genes specifically induced via the RANKL-Rac1 signaling pathway. Among identified targets, we show that RANKL selectively induces cyclooxygenase (COX) 2 expression via Rac1 that results in turn in production of prostaglandin E-2 (PGE(2)) in RAW 264.7 cells. By using transient transfection assays, we found that the -233/-206 region of the COX-2 promoter gene was critical for RANKL-induced promoter activity. This RANKL-responsive region contained an NF-kappa B site that, when mutated, completely abolished the induction of NF-kappa B DNA-binding activity by RANKL. Blockade of COX-2 by celecoxib inhibits differentiation of bone marrow-derived monocyte/macrophage precursor cells (BMMs) into tartrate-resistant acid phosphatase-positive (TRAP(+)) osteoclastic cells. This inhibition can be rescued by the addition of exogenous PGE(2), suggesting that COX-2-dependent PGE(2) induction by RANKL in osteoclast precursors is required for osteoclast differentiation.
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