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The Schizosaccharomyces pombe gene encoding gamma-glutamyl transpeptidase I is regulated by non-fermentable carbon sources and nitrogen starvationThe Schizosaccharomyces pombe Gene Encoding γ-Glutamyl Transpeptidase I Is Regulated by Non-fermentable Carbon Sources and Nitrogen Starvation

Other Titles
The Schizosaccharomyces pombe Gene Encoding γ-Glutamyl Transpeptidase I Is Regulated by Non-fermentable Carbon Sources and Nitrogen Starvation
Authors
Kim, HGPark, HJKang, HJLim, HWKim, KPark, EHAhn, KLim, CJ
Issue Date
Feb-2005
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Keywords
Schizosaccharomyces pombe; gamma-glutamyl transpeptidase; non-fermentable carbon sources; regulation; GGTI-lacZ fusion gene; Pap1; ethanol; acetate; nitrogen starvation
Citation
JOURNAL OF MICROBIOLOGY, v.43, no.1, pp 44 - 48
Pages
5
Journal Title
JOURNAL OF MICROBIOLOGY
Volume
43
Number
1
Start Page
44
End Page
48
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/15546
ISSN
1225-8873
1976-3794
Abstract
In our previous study, the first structural gene (GGTI) encoding gamma-glutamyl transpeptidase was cloned and characterized from the fission yeast Schizosaccharomyces pombe, and its transcription, using the GGTI-lacZ fusion gene, containing the 1,085 bp upstream region from the translational initiation point, was found to be enhanced by sodium nitroprusside and L-buthionine-(S,R)-sulfoximine (BSO). In the present work, regulation of the GGTI gene was further elucidated. Non-fermentable carbon sources, such as acetate and ethanol, markedly enhanced the synthesis of beta-galactosidase from the GGTI-lacZ fusion gene. However, its induction by non-fermentable carbon sources appeared to be independent of the presence of the Pap1 protein. Nitrogen starvation also gave rise to induction of GGTI gene expression in a Pap1-independent manner. The three additional fusion plasmids, carrying 754, 421 and 156 bp regions, were constructed. The sequence responsible for the induction by non-fermentable carbon sources and nitrogen starvation was identified to exist within a -421 bp region of the GGTI gene. Taken together, the S. pombe GGTI gene is regulated by non-fermentable carbon sources and nitrogen starvation.
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